时变参数神经电刺激系统的设计及其应用  

Design and application of neural electrical stimulation system with time-varying parameters

作　　者：杨刚生 封洲燕[1] 郑吕漂 王兆祥[1] YANG Gangsheng;FENG Zhouyan;ZHENG Lüpiao;WANG Zhaoxiang(Key Laboratory of Biomedical Engineering of Education Ministry,College of Biomedical Engineering and Instrument Science,Zhejiang University,Hangzhou 310027,P.R.China)

机构地区：[1]浙江大学生物医学工程与仪器科学学院生物医学工程教育部重点实验室,杭州310027

出　　处：《生物医学工程学杂志》2021年第6期1144-1153,共10页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30970753)。

摘　　要：目前,常规神经电刺激产品仅提供可预设参数的恒定刺激模式,而新型时变刺激模式是拓展神经电刺激产品临床应用的重要研究方向之一。为了便于在动物实验中施行时变的电刺激,本研究设计利用LabVIEW软件,控制通用的数据采集卡和电刺激器,构建了可输出各种实时变化的脉冲序列集成刺激系统。它可以产生具有均匀、正态、伽马和泊松等特定分布的时变脉冲间隔(IPI)脉冲序列,也可以产生其他任意时变IPI脉冲序列。此外,脉冲幅值、脉宽、双相脉冲相间延时和脉冲序列时长等参数可调。该刺激系统的性能测试结果显示,其输出脉冲序列各种参数的误差均小于1%。利用此系统生成间隔在5~10 ms范围内随机时变的脉冲序列,并用于大鼠海马脑区进行动物实验。实验结果显示,即使平均脉冲频率同样为130 Hz左右,随机变频刺激对于神经元群体的兴奋作用显著强于恒定频率刺激。总之,本文设计的刺激系统可为研究和开发新型神经电刺激模式提供有用的工具。Currently,commercial devices for electrical neural stimulations can only provide fixed stimulation paradigms with preset constant parameters,while the development of new stimulation paradigms with time-varying parameters has emerged as one of the important research directions for expanding clinical applications.To facilitate the performance of electrical stimulation paradigms with time-varying parameters in animal experiments,the present study developed a well-integrated stimulation system to output various pulse sequences by designing a LabVIEW software to control a general data acquisition card and an electrical stimulus isolator.The system was able to generate pulse sequences with inter-pulse-intervals(IPI)randomly varying in real time with specific distributions such as uniform distribution,normal distribution,gamma distribution and Poisson distribution.It was also able to generate pulse sequences with arbitrary time-varying IPIs.In addition,the pulse parameters,including pulse amplitude,pulse width,interphase delay of biphasic pulse and duration of pulse sequence,were adjustable.The results of performance tests of the stimulation system showed that the errors of the parameters of pulse sequences output by the system were all less than 1%.By utilizing the stimulation system,pulse sequences with IPI randomly varying in the range of 5~10 ms were generated and applied in rat hippocampal regions for animal experiments.The experimental results showed that,even with a same mean pulse frequency of~130 Hz,for neuronal populations,the excitatory effect of stimulations with randomly varying IPIs was significantly greater than the effect of stimulations with fixed IPIs.In conclusion,the stimulation system designed here may provide a useful tool for the researches and the development of new paradigms of neural electrical stimulations.

关 键 词：神经电刺激 时变刺激 随机脉冲序列 大鼠海马区 

分 类 号：TH77[机械工程—仪器科学与技术] TN911.7[机械工程—精密仪器及机械]