转化生长因子β信号通路相关因子在幼兔激素性股骨头坏死模型中的表达及意义  

The role and significance of the transforming growth factor-β signaling pathway in the model of steroid-induced osteonecrosis of the femoral head in young rabbits

作　　者：王楠锴 刘浩 张天久 聂淼桔 胡选琛 俞松 Wang Nankai;Liu Hao;Zhang Tianjiu;Nie Miaoju;Hu Xuanchen;Yu Song(Department of Pediatric Orthopedic Surgery,Affiliated Hospital of Zunyi Medical University,Zunyi 563003,Guizhou Province,China)

机构地区：[1]遵义医科大学附属医院小儿矫形外科,贵州遵义563003

出　　处：《中华实用儿科临床杂志》2021年第23期1811-1814,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：贵州省科技计划项目(黔科合基础(2018)1430,黔科合支撑(2021)一般076)。

摘　　要：目的探讨转化生长因子β(TGF-β)信号通路与幼兔激素性股骨头坏死的关系。方法选取8周龄新西兰白兔60只,体质量1.5~2.0 kg。按随机数表法将实验动物分为2组:实验组(48只),醋酸泼尼松龙7.5 mg/kg双侧臀肌交替注射,每周2次,共注射8周,其中造模成功的设为发病组,造模未成功的设为未发病组;对照组(12只),于同样部位相同频次注射等体积9 g/L盐水,为预防感染,每周注射1次青霉素钠(50000 U/只)。第8周注射完后行CT检查并处死所有实验动物,取出股骨头。酶联免疫吸附实验(ELISA)检测TGF-β信号通路相关因子TGF-β1、TGF-β2、Smad2、Smad3在股骨头中的表达,实时荧光定量PCR(qPCR)检测下游矮小相关转录因子2(Runx2)在股骨头中的表达,比较各组中相关因子的表达差异。结果实验组造模过程中处死6只,存活32只,发病6只(发病组),未发病26只(未发病组),造模成功率为18.75%(6/32只)。ELISA示:对照组、未发病组及发病组的TGF-β1表达量分别为(77.12±14.62)ng/L、(90.17±11.90)ng/L、(126.14±25.66)ng/L,发病组与未发病组及对照组比较,差异均有统计学意义(t=3.35、4.24,均P<0.05);对照组、未发病组及发病组的TGF-β2表达量分别为(74.54±7.63)ng/L、(89.24±9.51)ng/L、(109.74±16.45)ng/L,发病组与未发病组及对照组比较,差异均有统计学意义(t=4.12、5.65,均P<0.01);对照组、未发病组及发病组的Smad2表达量分别为(17.74±2.72)μg/L、(23.82±3.58)μg/L、(31.28±3.88)μg/L,发病组与未发病组及对照组比较,差异均有统计学意义(t=4.54、7.99,均P<0.01);对照组、未发病组及发病组的Smad3表达量分别为(1.76±0.52)μg/L、(2.39±0.45)μg/L、(3.53±0.47)μg/L,发病组与未发病组及对照组比较,差异均有统计学意义(t=5.60、6.71,均P<0.01)。qPCR示:Runx2在对照组、未发病组及发病组的相对表达量分别为1.02±0.17、1.27±0.14、1.72±0.11,发病组与未发病组及对照组比较,差异均有统�Objective To explore the relationship between the transforming growth factor-β(TGF-β)signaling pathway and steroid-induced osteonecrosis of the femoral head in young rabbits.Methods Sixty 8-week-old rabbits weighing 1.5-2.0 kg were randomly divided into steroid injection group(48 cases)and control group(12 cases).Rabbits in the former group were injected with Prednisolone Acetate 7.5 mg/kg into bilateral gluteal muscles twice a week for 8 weeks,and those with successful modeling were included in the disease group;otherwise,they were included in the non-disease group.Rabbits in control group were similarly injected with the same volume of 9 g/L saline.Penicillin sodium 50000 U/rabbit was injected once a week for preventing infection.After 8 weeks of injection,CT was performed in all the experimental animals.They were then sacrificed for collecting bilateral femoral heads.Expression levels of TGF-β1,TGF-β2,Smad2 and Smad3 in the femoral head were detected by enzyme linked immunosorbent assay(ELISA),and the mRNA level of Runx2 in the femoral head was detected by quantitative real-time PCR(qPCR),the expression differences of related factors in each group were compared.Results In steroid injection group(48 cases),6 rabbits were sacrificed,and 32 survived,involving 6/32 cases(18.75%)experimental animals with positive avascular necrosis(disease group),and 26 negative ones(non-disease group).ELISA data showed that expression levels of TGF-β1 in control group,non-disease group and disease group were(77.12±14.62)ng/L,(90.17±11.90)ng/L and(126.14±25.66)ng/L,respectively(t=3.35,4.24,all P<0.05).The expression levels of TGF-β2 in control group,non-disease group and disease group were(74.54±7.63)ng/L,(89.24±9.51)ng/L and(109.74±16.45)ng/L,respectively(t=4.12,5.65,all P<0.01).The expression levels of Smad2 in control group,non-disease group and disease group were(17.74±2.72)μg/L,(23.82±3.58)μg/L and(31.28±3.88)μg/L,respectively(t=4.54,7.99,all P<0.01).The expression levels of Smad3 in control group,non-disease

关 键 词：激素性股骨头坏死 动物模型/幼兔 转化生长因子β信号通路 

分 类 号：R681.8[医药卫生—骨科学] R-332[医药卫生—外科学]