家蚕BmHsp19.5基因的鉴定及在滞育卵中的表达分析  

Identification and Expression Analysis of BmHsp19.5 Gene in Diapause Eggs of Bombyx mori

作　　者：龚竞[1] 尚杉 郑茜 于新波[1] 朱勇[1] GONG Jing;SHANG Shan;ZHENG Xi;YU Xinbo;ZHU Yong(Key Laboratory of Sericultural Biology and Genetic Breeding,Ministry of Agriculture/College of Sericulture,Textile and Biomass Sciences,Southwest University,Chongqing 400715,China)

机构地区：[1]农业农村部蚕桑生物学与遗传育种重点实验室/西南大学蚕桑纺织与生物质科学学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2022年第1期57-64,共8页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目(31802139,31872429);重庆市自然科学基金项目(cstc2021jcyj-msxmX1166).

摘　　要：基于前期转录组学研究结果,从家蚕滞育卵和发育蚕卵中筛选出差异表达基因家蚕BmHsp19.5.为了进一步了解该基因的序列信息、表达特征以及在家蚕卵滞育过程中的功能,本文采用基因克隆、生物信息学方法以及qPCR检测等技术手段对该基因进行分析.结果表明:家蚕BmHsp19.5基因的ORF(open reading frame)框全长504 bp,共编码167个氨基酸,其蛋白的理论相对分子质量为1.95×10^(4).该蛋白65-157位氨基酸为Hsp20功能结构域,符合小热激蛋白家族结构特征.该基因在滞育卵和非滞育卵中表达量存在显著差异,在滞育卵中呈上调表达,且在第3 d达到最大值,推测该基因与滞育过程有关,可能在家蚕滞育进入时发挥了重要作用.Based on the results of previous transcriptomics research,the differentially expressed BmHsp19.5 gene was screened from the diapause eggs and developing silkworm eggs of the silkworm.To further understand the sequence information,expression characteristics and function of the BmHsp19.5 gene during the diapause process,we used gene cloning,bioinformatics methods and qPCR detection to analyze the gene.The results showed that the ORF of the BmHsp19.5 gene was 504 bp in length,encoding a total of 167 amino acids,and the theoretical molecular weight of the protein was 1.95×10^(4).The 65-157 amino acid fragment of the protein was Hsp20 functional domain,which was consistent with the structural characteristics of small heat shock protein family.The expression level of the gene in diapause and non-diapause eggs was significantly different,and the expression of the gene was up-regulated in diapause eggs,and reached the maximum on the 3rd day.Taken together,it is speculated that BmHsp19.5 gene is related to diapause process and may play an important role in diapause entry of silkworm.

关 键 词：家蚕 BmHsp19.5基因 滞育 表达分析 

分 类 号：S882[农业科学—特种经济动物饲养]