促渗剂对淫羊藿苷体外经皮渗透特性影响的研究  

Effect of penetration enhancers on percutaneous penetration characteristics of icariin in vitro

作　　者：何红杰 王继龙[1] 刘晓霞[1] 冉鑫 魏舒畅 冯晓莉[1] 万玲娟 HE Hong-jie;WANG Ji-long;LIU Xiao-xia;RAN Xin;WEI Shu-chang;FENG Xiao-li;WAN Ling-juan(Gansu University of Chinese Medicine,Lanzhou 730000,China;Research Center of Traditional Chinese Medicine Pharmaceutical Technology and Engineering of Gansu Province,Lanzhou 730000 China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃省中药制药工艺工程研究中心,甘肃兰州730000

出　　处：《时珍国医国药》2021年第11期2661-2663,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(82060721);敦煌医学与转化教育部重点实验室项目(DHYX20-12)。

摘　　要：目的研究不同透皮促渗剂对淫羊藿苷体外经皮渗透的影响,筛选出最佳促渗剂。方法选用氮酮、薄荷脑、冰片为促渗剂,采用Franz扩散池法,考察单一促渗剂及三元复合促渗剂对淫羊藿苷体外透皮吸收的影响,采用正交设计,选用L_(9)(3^(4))正交试验表,以淫羊藿苷累积渗透量为指标,优选出三元复合促渗剂。以雄性小鼠离体皮肤作为渗透屏障,含20%乙醇的PBS溶液为接收液,HPLC法测定淫羊藿苷的含量。结果促渗剂以1%氮酮、2%冰片及2%薄荷脑为组合时促渗效果最佳。结论三元复合促渗剂可显著提高淫羊藿苷的经皮渗透性,为后续将淫羊藿及以淫羊藿为主要的方剂制备成经皮制剂提供促渗物质。Objective To study the effects of different transdermal enhancers on the percutaneous penetration of icariin in vitro, and select the best enhancer. Methods Azone, menthol and borneol were selected as penetration enhancers. Franz diffusion cell method was used to investigate the effects of single penetration enhancer and ternary composite penetration enhancer on the percutaneous absorption of icariin in vitro. Orthogonal design was adopted, L_(9)(3^(4)) orthogonal test table was selected, and the cumulative penetration amount of icariin was taken as the index to screen out the best ternary penetration enhancer. The content of icariin was determined by HPLC with the isolated skin of male mice as permeation barrier and PBS solution containing 20% ethanol as receiving solution. Results The combination of 1% azone, 2% borneol and 2% menthol had the best permeation promoting effect. Conclusion Ternary penetration enhancers could significantly improve the transdermal permeability of icariin, and provide penetration enhancers for the subsequent preparation of epimedium and epimedium-containing prescriptions into transdermal preparations.

关 键 词：经皮渗透 促渗剂 正交设计 淫羊藿 

分 类 号：R285.5[医药卫生—中药学]