肉桂HPLC指纹图谱与颜色相关性及化学模式识别分析  

Correlation between HPLC fingerprint and color and chemical identification pattern analysis of Cinnamomi Cortex

作　　者：刁元元 邱智东[1] 张欣舒 杨净尧 高寒 张宇航 贾艾玲[1,2] DIAO Yuan-yuan;QIU Zhi-dong;ZHANG Xin-shu;YANG Jing-yao;GAO Han;ZHANG Yu-hang;JIA Ai­ling(Changchun University of Chinese Medicine,Changchun Jilin 130117,China;Chengdu University of Traditional Chinese Medicine,Chengdu Sichuan 611137,China)

机构地区：[1]长春中医药大学药学院,吉林长春130117 [2]成都中医药大学药学院,四川成都611137

出　　处：《时珍国医国药》2021年第11期2676-2680,共5页Lishizhen Medicine and Materia Medica Research

基　　金：吉林省教育厅科学研究项目(JJKH20210987KJ);经典名方保元汤合作开发项目(20180309112378)。

摘　　要：目的探讨肉桂去除粗皮的合理性、指纹图谱与颜色相关性及化学模式识别分析。方法按照《中华人民共和国药典》2020年版肉桂项下方法测定桂皮醛、挥发油的含量;采用色度仪测定肉桂药材粉末的色度值;采用HPLC色谱法建立肉桂指纹图谱;采用SPSS 21.0软件进行相关性分析;采用聚类分析、主成分分析、正交偏最小二乘判别分析等进行化学模式识别研究。结果除去粗皮后,桂皮醛及挥发油含量增高;18批肉桂药材指纹图谱共建立9个共有峰,指认4个色谱峰,分别为香豆素、肉桂醇、肉桂酸、桂皮醛;香豆素含量与L;、a;、b;值均无显著相关性,肉桂醇含量与a;、b;值均呈显著负相关,肉桂酸含量与a;值呈显著负相关,与b;值呈极显著负相关;桂皮醛含量与b*值呈显著负相关;3种分析方式将18批肉桂分为两类,大体呈产区相关性。结论肉桂去除粗皮具有合理性,色度值与HPLC指纹图谱具有相关性,结合化学模式识别分析可综合评价肉桂药材质量。Objective To investigate the reasonableness of the removal of rough peel in Cinnamomi Cortex and the correlation between HPLC fingerprint and color and chemical identification pattern analysis of Cinnamomi Cortex.Methods The contents of cinnamc aldehyde and volatile oil were determined according to the method of cinnamon in 2015 Edition of Chinese Pharmacopoeia. The color values of Cinnamomi Cortex was determined by colorimeter. The fingerprint of cinnamon was established by HPLC. SPSS 21.0 software was used for correlation analysis. Cluster analysis, principal component analysis and orthogonal partial least squares discriminant analysis were used to study chemical pattern recognition. Results The content of cinnamaldehyde and essential oil increased after removing the crude peel. 9 common peaks were established in 18 batches of cinnamon, and 4 peaks were identified, namely coumarin, cinnamyl alcohol, cinnamic acid and cinnamic aldehyde. There was no significant correlation between coumarin content and values of L;, a;and b;. while there was a significant negative correlation between cinnamol content and values of a;and b;. The content of cinnamic acid was significantly negatively correlated with value of a;and a very significantly negatively correlated with value of b;. There was a significant negative correlation between cinnamaldehyde content and value of b;. The 18 batches of Cinnamomi Cortex were divided into 2 categories by 3 analysis methods, which showed the correlation of producing areas. Conclusion It is reasonable for cinnamon to remove the rough skin. The chromaticity value was correlated with HPLC fingerprint, and the quality of Cinnamomi Cortex could be evaluated synthetically by chemical pattern recognition analysis.

关 键 词：肉桂 HPLC 指纹图谱 色度值 化学模式识别分析 

分 类 号：R284.1[医药卫生—中药学]