不同黄精九制炮制品红外指纹图谱研究  

Study on infrared Fingerprint of different Processed products of Jiuzhi Polygonati Rhizome

作　　者：江露娟 陈诗曼 韦灿蓉 王国敏 银胜高[1] JIANG Lu-juan;CHEN Shi-man;WEI Can-rong;WANG Guo-min;YIN Sheng-gao(Guangxi University of Chinese Medicine,Nanning Guangxi,530001 China)

机构地区：[1]广西中医药大学,广西南宁530001

出　　处：《时珍国医国药》2021年第11期2685-2687,共3页Lishizhen Medicine and Materia Medica Research

基　　金：中国-东盟传统医药发展研究中心开发课题(05J1601603;0501801702)。

摘　　要：目的对不同黄精九制品进行红外指纹图谱研究。方法采用傅立叶变换红外光谱法对12批三种不同黄精九制品进行红外指纹图谱检测,用红外光谱软件Omnic 7.0求出平均图谱,并对各样品间的相似度及红外指纹图谱进行分析。结果获得12批不同黄精炮制品红外指纹图谱共有峰有3384 cm^(-1)、2931 cm^(-1)、1635 cm^(-1)、1414 cm^(-1)、1340 cm^(-1)、1320 cm^(-1)、1249 cm^(-1)、1146 cm^(-1)、1061 cm^(-1)、922 cm^(-1)、895 cm^(-1)、868 cm^(-1)、818 cm^(-1)、779 cm^(-1)、700 cm^(-1)、620 cm^(-1)、523 cm^(-1)、422cm^(-1)。各样品间IR相似度在0.9159~0.9890范围,因此拟定九制黄精的IR相似度阈值为0.90。三种九制黄精炮制品的红外光谱鉴别特征体征为:在880cm^(-1)和685cm^(-1)两个鉴别峰附近,多花黄精均为单峰;黄精均为双峰;滇黄精在880cm^(-1)附近为单峰,685cm^(-1)附近为双峰,此外黄精还有个特有的1367cm^(-1)鉴别峰。结论该实验研究方法具有专属性强、灵敏度高、取样量小、简便迅速等特点,红外光谱可以分析不同黄精炮制品的相似度和图谱特征峰的变化规律,可为不同黄精炮制品的快速鉴别提供参考。Objective To study the infrared fingerprint of the ninth steamed product of different Polygonati Rhizome. Methods Fourier transform infrared spectroscopy was used to detect infrared fingerprints of 12 batches of the ninth steamed product of 3 different Polygonati Rhizome. Use the infrared spectroscopy software Omnic 7.0 to obtain the average spectrum, and analyze the similarity between the samples, and the infrared fingerprint spectrum.Results The infrared fingerprints of 12 batches of different Polygonati Rhizome processed products have common peaks of 3384 cm^(-1),2931 cm^(-1), 1635 cm^(-1), 1414 cm^(-1), 1340 cm^(-1), 1320 cm^(-1), 1249 cm^(-1), 1146 cm^(-1), 1061 cm^(-1), 922 cm^(-1), 895 cm^(-1), 868 cm^(-1), 818 cm^(-1), 779 cm^(-1), 700 cm^(-1), 620 cm^(-1), 523 cm^(-1)and 422 cm^(-1). The IR similarity between samples was in the range of 0.9159 to 0.9890, so the IR similarity threshold of Jiuzhi Polygonati Rhizome was proposed to be 0.90. The infrared spectroscopic identification characteristics of the ninth steamed product of 3 different Polygonati Rhizome were: near the two identification peaks of 880 cm^(-1)and 685 cm^(-1), Polygonatum cyrtonema Hua. was single peak;Polygonatum sibiricum Red. was double-peaked;Polygonatum kingianum Coll.et Hemsl has a single peak near 880 cm^(-1)and a double peak near 685 cm^(-1). In addition, Polygonatum sibiricum Red. has a unique identification peak at 1367 cm^(-1). Conclusion The experimental research method has the characteristics of strong specificity, high sensitivity, small sampling volume, simple and rapid, etc. Infrared spectroscopy could analyze the similarity of different Polygonati Rhizome processed products and the change rule of the characteristic peaks of the spectrum, which could provide a reference for the rapid identification of different Polygonati Rhizome processed products.

关 键 词：黄精 多花黄精 滇黄精 九制黄精 IR 

分 类 号：R284.1[医药卫生—中药学]