䓛与鲱鱼精子DNA相互作用及其影响因素的光谱学分析  

Spectroscopic Analysis on the Interaction of Chrysene With Herring Sperm DNA and Its Influence Factors

作　　者：吕嘉楠 李军生[1] 黄国霞[1] 阎柳娟[1] 马纪 Lü Jia-nan;LI Jun-sheng;HUANG Guo-xia;YAN Liu-juan;MA Ji(Guangxi Key Laboratory of Green Processing of Sugar Resources,Guangxi University of Science and Technology,Liuzhou 545006,China)

机构地区：[1]广西科技大学广西糖资源绿色加工重点实验室,广西柳州545006

出　　处：《光谱学与光谱分析》2022年第1期210-214,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(21966008);广西自然科学基金项目(2018GXNSFDA281030,2020GXNSFAA159021)资助。

摘　　要：利用紫外-可见吸收光谱和共振光散射法研究䓛(chrysene,CHR)与鲱鱼精子DNA的相互作用,通过计算其DNA结合饱和值来评估CHR对DNA的结合能力。此外,还分别考察了温度,pH,氯化钠,氯化钙,维生素C,十二烷基磺酸钠等环境因素对CHR与DNA相互作用的影响,为下一步利用DNA构建消除CHR的方法奠定基础。紫外可见光谱显示,加入DNA后,CHR的最大吸收峰出现减色效应且伴随有红移现象,这说明CHR与DNA的相互作用属于嵌插结合模式。共振光散射光谱结果表明CHR在468 nm处出现稳定的共振光散射峰。当CHR的浓度为2.59×10^(-6) mol·L^(-1)时,其饱和DNA浓度为3.08×10^(-6) mol·L^(-1),CHR的DNA结合饱和值在25℃时为0.84,而当pH=7.40,30℃时,CHR的DNA结合饱和值则达到0.94。在此条件下,氯化钠,氯化钙,维生素C,十二烷基磺酸钠对CHR与DNA相互作用的影响也不同,相应CHR的DNA结合饱和值分别为0.84,0.76,1.25和1.07。以30℃时CHR的DNA结合饱和值为基准,各环境共存物下的相应DNA结合饱和值变化率分别为-10%,-19%,33%和14%,说明阳离子的存在会抑制CHR与DNA的结合,而一定浓度的维生素C和十二烷基磺酸钠则对CHR与DNA的结合有协同促进作用。本研究结果可为建立新的基于DNA嵌插的CHR去除法提供参考。The interaction between chrysene(CHR)and herring sperm DNA(hsDNA)was studied by UV-Visible absorption spectra(UV-Vis)and resonance light scattering(RLS)Spectrometry.Ability binding with DNA of CHR was evaluated by calculating the saturation value binding with DNA of CHR.In addition,the effects of temperature,pH,sodium chloride,calcium chloride,vitamin C,sodium dodecyl sulfate on the interaction between CHR and DNA respectively were respectively investigated,which laid the foundation for establishing a method to eliminate CHR by DNA in the future.UV-Vis spectra showed that after DNA was added,the maximum absorption peak of CHR appeared hypochromic effect and accompanied by a red-shift,suggesting that CHR interacted with DNA in an intercalation mode.The RLS spectra showed that CHR had a stable RLS peak at 468 nm.When the concentration of CHR was 2.59×10^(-6 )mol·L^(-1),the saturated concentration of binding with DNA was 3.08×10^(-6) mol·L^(-1),saturation value binding with DNA of CHR was 0.84 at 25℃,and saturation value binding with DNA of CHR was 0.94 at pH 7.40 at 30℃.Under this condition,sodium chloride,calcium chloride,vitamin C,sodium dodecyl sulfate also had different effects on the interaction of CHR with DNA.Saturated value binding with DNA of CHR were 0.84,0.76,1.25,1.07 respectively.Their change rate of saturation value binding with DNA with CHR-DNA-30℃as a standard are-10%,-19%,33%and 14%,which showed that the presence of cationic can inhibit the combination of CHR with DNA,while a certain concentration of vitamin C and sodium dodecyl sulfonate had a synergistic promotion effect on the interaction of CHR with DNA.This study could provide a reference for establishing a new CHR removal method via DNA based on DNA-intercalation.

关 键 词：䓛 DNA 相互作用 光谱法 结合饱和值 影响因素 

分 类 号：O657.3[理学—分析化学]