TMA联检反应性而鉴别非反应性献血者标本HBV血清学标志物分析  

Analysis of HBV serological markers of donor blood samples implicated in TMA triplex + but discriminatory test-samples

作　　者：李素萍[1] 李明睿 王恺 王婷婷 吕晴 倪德群 王婷 谢欣 程卫芳[1] LI Suping;LI Mingrui;WANG Kai;WANG Tingting;LÜ Qin;NI Dequn;WANG Ting;XIE Xin;CHENG Weifang(Anhui Blood Center,Hefei 230031,China)

机构地区：[1]安徽省血液中心,安徽合肥230031

出　　处：《中国输血杂志》2021年第11期1228-1231,共4页Chinese Journal of Blood Transfusion

基　　金：合肥市卫健委2019年应用医学研究项目(hwj2019zd018)。

摘　　要：目的探讨TMA核酸检测联检反应性而鉴别非反应性献血者标本HBV感染状态,是否存在低浓度HBV DNA。方法 2020年1月~2021年3月期间用Procleix Panther核酸检测系统共检测51 996份标本,收集TMA核酸检测联检反应性而鉴别非反应性献血者标本86份,采用电化学发光法检测HBV血清学标志物(HBsAg、抗-HBs、抗-HBc、HBeAg、抗-HBe),对部分鉴别非反应性献血者标本进行罗氏核酸单检。结果 86份TMA核酸检测联检反应性鉴别非反应性献血者标本中:抗-HBc阳性率为89.53%(77/86),抗-HBe阳性率为27.90%(24/86),抗-HBs阳性率为65.12%(56/86)。抗-HBs/抗-HBe/抗-HBc阳性15份,抗-HBs/抗-HBc阳性34份,抗-HBs/抗-HBe阳性1份,抗-HBe/抗-HBc阳性8份,单独抗-HBs阳性6份,单独抗-HBc阳性20份,HBV血清学标志物阳性率97.67%(84/86)。10份标本经罗氏单检定性检测HBV DNA有5份,1份HBV DNA定量<20 IU/mL。结论 TMA核酸检测联检反应性而鉴别非反应性献血者多为低浓度的隐匿性乙肝感染。Objective To explore the status of HBV infection and low viral load of HBV DNA in blood donor samples implicated in TMA triplex reactive but discriminatory test non-reactive samples.Methods A total of 51 996 samples were detected by Procleix Panther nucleic acid detection(NAT) system from January 2020 to March 2021, and 86 of them were TMA triplex reactive but discriminatory test non-reactive. HBV serological markers(HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc) were detected by electrochemiluminescence. Single-donation(ID) NAT was conducted in some TMA triplex +/discriminatory test-samples using Roche NAT.Results Out of 86 TMA triplex +/discriminatory test-samples, anti-HBc were positive in 89.53%(77/86), anti-HBe positive in 27.90%(24/86) and anti-HBs positive in 65.12%(56/86). 15 donors carried anti-HBs, anti-HBe and anti-HBc, 34 both anti-HBs and anti-HBc, 1 both anti-HBs/anti-HBe, 8 both anti-HBe/anti-HBc, 6 solo anti-HBs, and 20 solo anti-HBc. The positive rate of HBV serological markers was 97.67%(84/86). HBV DNA in 5 out of the 10 samples was qualitatively detected by Roche ID NAT, and one of them presented HBV DNA < 20 IU/mL. Conclusion Most TMA triplex +/discriminatory test-samples were occult hepatitis B infection.

关 键 词：献血者 乙型肝炎病毒 核酸检测 血清学标志物 

分 类 号：R446[医药卫生—诊断学] R512.612[医药卫生—临床医学]