利用过冷方法从液态血浆制备冷沉淀凝血因子的研究  

Preparation of cryoprecipitate coagulation factor from liquid-state plasma by supercooling method

作　　者：康炜[1] 李德龙 王东[1] 周曼华 李娟娟 KANG Wei;LI Delong;WANG Dong;ZHOU Manhua;LI Juanjuan(Dalian Blood Center,Dalian 116001,China;Dalian Medi-cal University)

机构地区：[1]大连市血液中心,辽宁大连116001 [2]大连医科大学

出　　处：《中国输血杂志》2021年第11期1261-1262,共2页Chinese Journal of Blood Transfusion

摘　　要：目的利用过冷方法建立冷沉淀凝血因子全新制备方法。方法新鲜液体血浆在-11℃~-13℃静止保存一段时间,由于纯净液体所具有的过冷状态特点,血浆仍保持液体状态而不会结冰,但部分凝血因子等会因为温度降低而生成固体冷不溶物,虹吸法分离液体血浆,即可获得血浆冷沉淀。结果 50例新鲜液体血浆过冷状态静止保存16 h,制得冷不溶物中纤维蛋白原平均含量(186.02±22.72)mg,平均回收率(37.51±7.42)%;凝血因子Ⅷ含量平均(104.66±22.88)IU,平均回收率(46.62±5.58)%。结论血浆冷沉淀凝血因子需重新定义,并非仅能由新鲜冰冻血浆低温融化制得,也可以由液体血浆直接制得;新鲜液体血浆过冷方法制备冷沉淀凝血因子操作简单,质量稳定,符合《全血成分血质量要求》GB18469-2012标准。Objective To establish a novel preparation method of cryoprecipitate coagulation factor from overcooled liquid-state plasma. Methods The fresh liquid plasma was kept at-11℃ to-13℃ for a period of time. It can remain in the liquid state with some coagulation factors generated due to supercooling. Then cryoprecipitate can be obtained from the liquid plasma by siphon method. Results The average fibrinogen content yielded in cryoprecipitate, prepared from 50 samples of 16-hour-stored fresh liquid plasma, was(186.02±22.72) mg, with the average recovery rate of(37.51±7.42) %, and the average content of coagulation FⅧ was(104.66±22.88) IU, with the average recovery rate of(46.62±5.58) %. Conclusion The cryoprecipitate coagulation factors could be obtained not only from fresh frozen-thawed plasma, but also from overcooled liquid plasma which is simple and stable, also meets the requirements of relative standards.

关 键 词：过冷方法 冷沉淀凝血因子 纤维蛋白原 凝血因子Ⅷ 

分 类 号：R457[医药卫生—治疗学]