实时荧光定量PCR检测妊娠晚期孕妇B族链球菌感染的临床价值分析  

Analysis of the clinical value of real-time fluorescence quantitative PCR in the detection of group B streptococcus infection in pregnant women in late pregnancy

作　　者：马倩 MA Qian(Department of Genetics,Shenyang Maternal and Child Health Hospital,Shenyang 110014,China)

机构地区：[1]沈阳市妇幼保健院遗传科,110014

出　　处：《中国实用医药》2021年第34期53-55,共3页China Practical Medicine

摘　　要：目的分析实时荧光定量聚合酶链反应(PCR)检测妊娠晚期孕妇B族链球菌(GBS)感染的临床价值。方法3721例接受B族链球菌感染检测的孕晚期孕妇,经采集阴道分泌物及直肠分泌物后均接受常规细菌培养及实时荧光定量PCR检测。对比细菌培养与实时荧光定量PCR检测结果;不同年龄段孕妇B族链球菌阳性检出率;B族链球菌阴性、阳性孕妇妊娠结局。结果实时荧光定量PCR检测阴道分泌物、直肠分泌物阳性检出率分别为9.00%、9.16%,均高于细菌培养检测的7.28%、7.77%,差异具有统计学意义(P<0.05)。B族链球菌感染发生率随孕妇年龄增加上升,不同年龄段孕妇B族链球菌阳性检出率对比差异具有统计学意义(P<0.05)。B族链球菌阳性孕妇早产、胎膜早破、宫内感染、胎儿窘迫及新生儿感染发生率分别为28.74%、26.98%、16.72%、19.94%、16.13%,均高于B族链球菌阴性孕妇的2.22%、6.01%、0、7.13%、2.57%,差异有统计学意义(P<0.05)。结论实时荧光定量PCR检测对妊娠晚期孕妇B族链球菌感染具有显著应用价值,需在年龄较大孕妇中加强检测力度,且需在确认检验结果后及时予以干预,降低不良妊娠结局风险,保障母婴安全。Objective To analyze the clinical value of real-time fluorescence quantitative polymerase chain reaction(PCR)in the detection of group B streptococcus(GBS)infection in pregnant women in late pregnancy.Methods There were 3721 pregnant women in late pregnancy who were tested for group B streptococcus infection,and their vaginal secretions and rectal secretions were collected and then subjected to conventional bacterial culture and real-time fluorescence quantitative PCR assay.Comparison was made on test results of bacterial culture and real-time fluorescence quantitative PCR assay;positive detection rate of group B streptococcus in pregnant women of different ages;pregnancy outcome of group B streptococcus negative and positive pregnant women.Results The positive detection rates of vaginal secretions and rectal secretions by real-time fluorescent quantitative PCR were 9.00%and 9.16%,respectively,which were higher than 7.28%and 7.77%of bacterial culture,and the difference was statistically significant(P<0.05).The incidence of group B streptococcus infections increased with maternal age.There was no statistically significant difference in positive detection rate of group B streptococcus in pregnant women of different ages(P<0.05).The incidence of premature delivery,premature rupture of membranes,intrauterine infection,fetal distress and neonatal infection in pregnant women with positive group B streptococcus were 28.74%,26.98%,16.72%,19.94%,and 16.13%,respectively,which were higher than 2.22%,6.01%,0,7.13%,2.57%,and the difference was statistically significant(P<0.05).Conclusion Real-time fluorescence quantitative PCR assay is of great value for the detection of group B streptococcus infection in late pregnancy.It is necessary to strengthen the detection in older pregnant women,and intervene immediately after confirming the test results,so as to reduce the risk of adverse pregnancy outcome and ensure the safety of mother and baby.

关 键 词：实时荧光定量聚合酶链反应 妊娠晚期 B族链球菌感染 临床价值 

分 类 号：R714.251[医药卫生—妇产科学]