白洋淀野生大豆GsGST基因的克隆与鉴定  

Cloning and Identification of GsGST Gene in Wild Soybean(Glycine soja)from Baiyangdian Lake

作　　者：封润霞 李雅琳 赵婕 姚莹 刘建凤[1] Feng Runxia;Li Yalin;Zhao Jie;Yao Ying;Liu Jianfeng(College of Life Science,Hebei University,Baoding,071002)

机构地区：[1]河北大学生命科学学院,保定071002

出　　处：《分子植物育种》2021年第23期7673-7679,共7页Molecular Plant Breeding

基　　金：河北大学研究生创新项目(hbu2020ss036)资助。

摘　　要：为进一步解析白洋淀野生大豆抵御盐胁迫的分子机制,本研究从白洋淀不同区域野生大豆种质资源中筛选获得高抗(salt tolerance,ST)与低抗(salt sensitive,SS)耐盐性不同的试验材料,并通过克隆获得野生大豆GsGST基因的开放阅读框序列(open reading frame,ORF),并对其编码蛋白进行了性质分析、结构预测及同源进化分析,同时对该基因表达模式进行了鉴定。研究结果表明,GsGST基因含有660 bp开放阅读框,编码219个氨基酸,α-螺旋和无规则卷曲是其整体蛋白质结构中的主要组成结构元件;野生大豆Gs GST蛋白具有亲水性,含有跨膜结构,位于细胞质中;同时该基因编码的蛋白与野生大豆Tau类谷胱甘肽S-转移酶(GsGSTU)的同源性较高,达到99.54%。在高盐(NaCl)非生物因子胁迫处理下,GsGST基因表现明显的上调表达趋势。本研究结果为白洋淀野生大豆的耐盐机制提供理论基础,为培育常规耐盐大豆新材料提供种质资源与关键基因。To analyz e the molecular mechanism of resistance to salt stress in Glycine soja,in this study,salt tolerance(ST)and salt sensitive(SS)of Glycine soja germplasm were selected from different areas of Baiyangdian Lake.The open reading frame sequence of GsGST gene was obtained from Glycine soja.And the properties,structure prediction,homology evolution and gene expression pattern of the protein were analyzed.The results showed that Gs GST gene contains 660 bp of open reading frame,encoding 219 amino acids.Theα-helix and random coil were the main structural elements basis for Gs GST protein structure.Gs GST protein was hydrophilic,with transmembrane structure,located in the cytoplasm.And this geneis high homology with Tau-class glutathione S-transferases GSGSTU,up to 99.54%.Under the abiotic stress treatment of high salt(NaCl),it was found that GsGST gene could participate in the response of salt stress,the gene showed up-regulated expression.The results provide theoretical germplasm resources for salt tolerance mechanism and key genes for the breeding new materials of conventional salt-tolerant soybean.

关 键 词：野生大豆(Glycine soja) 白洋淀 GsGST 生物信息学 非生物胁迫 

分 类 号：S565.1[农业科学—作物学]