不同种皮颜色大豆地方资源的遗传多样性  

Genetic Diversity of Soybean Landraces with Different Seed Coat Color

作　　者：王英男[1] 齐广勋[1] 赵洪锟[1] 袁翠平[1] 刘晓冬[1] 李玉秋[1] 王玉民[1] 董英山[1] Wang Yingnan;Qi Guangxun;Zhao Hongkun;Yuan Cuiping;Liu Xiaodong;Li Yuqiu;Wang Yumin;Dong Yingshan(Jilin Academy of Agricultural Sciences,Changchun,130033)

机构地区：[1]吉林省农业科学院,长春130033

出　　处：《分子植物育种》2021年第23期7984-7994,共11页Molecular Plant Breeding

基　　金：吉林省农业科技创新工程重大项目(CXGC2017ZD014);吉林省农业科技创新工程项目(C82230416)共同资助。

摘　　要：本研究利用60对SSR引物对来自吉林省的52份大豆地方品种(系)进行遗传多样性和亲缘关系分析。结果表明,60对SSR引物共检测到545个等位变异,平均每个SSR位点检测到的等位变异数为9.08个,变化范围为2~18个;60个SSR标记的基因多样性在0.4529~0.9210之间,平均值为0.7579;多态性信息含量在0.3562~0.9157之间,平均值为0.7288。按种皮色分组进行分析,结果表明黑豆和青豆的遗传多样性较高。聚类分析结果表明,52份大豆地方品种(系)可分为5个类群。分子方差分析表明11.27%的变异来自组间,黄色种皮大豆与黑色种皮大豆、淡绿色种皮大豆间的遗传分化系数分别为0.0788和0.0867,存在适度的遗传分化。本研究可为大豆地方品种资源的深入利用及黑豆和青豆品种的遗传改良提供参考信息。In this study,the genetic diversity of 52 soybean landraces was analyzed using 60 SSR(Simple sequence repeat)markers.The results showed that a total of 545 alleles were detected by 60 pairs of SSR primers,with a range of 2~18 alleles and an average of 9.08 alleles per SSR locus.The gene diversity of 60 SSR markers was0.4529~0.9210,with an average of 0.7579;The polymorphism information content was 0.3562~0.9157,with an average of 0.7288.The results showed that the genetic diversity of black and green soybeans was relatively high.The results of cluster analysis showed that 52 soybean landraces could be divided into 5 groups.AMOVA analysis showed that 11.27%of the genetic variation existed among groups.The Fst between yellow and black seed coat soybean,and the Fst between yellow and light green seed coat soybean were 0.0788 and 0.0867,respectively,indicating moderate genetic differentiation.This study can provide reference information for further utilization of soybean landraces and genetic improvement of black and green soybean varieties.

关 键 词：大豆 SSR标记 遗传多样性 聚类分析 

分 类 号：S565.1[农业科学—作物学]