比色法测定滇黄精的中性糖和酸性糖含量  

Determination of the neutral sugars and uronic acids in Polygonatum kingianum by Colorimetric method

作　　者：顾健[1] 羌苏锐 刘江云[3] GU Jian;QIANG Su-rui;LIU Jiang-yun(Nantong Health Branch of Jiangsu Union Technical Institute,Nantong 226007,China;不详)

机构地区：[1]江苏联合职业技术学院南通卫生分院,江苏南通226007 [2]南通大学附属医院药学部,江苏南通226001 [3]苏州大学医学部药学院,江苏苏州215123

出　　处：《中国处方药》2021年第12期20-22,共3页Journal of China Prescription Drug

基　　金：江苏省科技厅现代农业项目(BE20180322)。

摘　　要：目的间羟基联苯法与蒽酮-硫酸法联用测定滇黄精中性糖与酸性糖的含量。方法间羟基联苯法测定条件:四硼酸钠-硫酸溶液6.0 ml,加热6 min,加入1.5 mg/ml的间羟基联苯溶液100μl,显色40 min,于525 nm下测定吸光度。蒽酮-硫酸法测定条件:2.0 g/ml的硫酸-蒽酮溶液4.0 ml,沸水浴显色10 min,于625 nm下测定吸光度。结果采用上述方法,测定PK-F1~PK-F3三个样品的中性总糖含量(以葡萄糖计)依次为78.0%、66.9%、64.5%;测定PK-F1~PK-F3三个样品的糖醛酸含量(以半乳糖醛酸计)依次为28.3%、16.2%、4.93%。结论该法准确便捷,为滇黄精多糖的中性糖和酸性糖测定的有效方法,可联合测定滇黄精中性糖和酸性糖含量。Objective To determine the neutral sugars and uronic acids in Polygonatum kingianum by the hydroxy diphenyl chromogenic method assay and the anthrone-sulfuric acid chromogenic method assay simultaneously.Methods The methods for the hydroxy diphenyl chromogenic method assay:the dosage of Na_(2)B_(4)O_(7)-H_(2)SO_(4) was 6.0 ml,heat for 6 minutes,and m-hydroxy dip henyl was 100 ul.Time for heating and color development were 40 min,The detection wave length was 525 nm.The methods for the anthrone-sulfuric acid chromogenic method assay:the dosage of anthrone-sulfuric acid was 6.0 ml,time for heating and color development were 10 min.The detection wave length was 625 nm.Results By the above method,the content of neutral total sugar in the PK-F1~PK-F3 samples was 78.0%,66.9%and 64.5%,respectively,and the content of uronic acid in the PK-F1~PK-F3 samples was 28.3%,16.2%,4.93%,respectively.Conclusion The method is reliable and convenient,which can be used for the determination of the neutral sugars and uronic acids in Polygonatum kingianum simultaneously.

关 键 词：滇黄精 中性糖 糖醛酸 间羟基联苯法 蒽酮-硫酸法 含量 

分 类 号：R284.1[医药卫生—中药学]