基于网络药理学分析心宝丸治疗病态窦房结综合征的成分与机制  

Network-based pharmacological analysis of the components and mechanisms of Xinbao Pills in the treatment of pathological sinus node syndrome

作　　者：赵慧敏 邢方凯 杨昌林[1] ZHAO Huimin;XING Fangkai;YANG Changlin(Department of Senior Cadre one,Ninety-seventh Hospital of the Joint Logistics and Security Forces of the Chinese People′s Liberation Army,Yantai,Shandong,264001,China;71217th Army of the Chinese People′s Liberation Army,Yantai,Shandong 266600,China)

机构地区：[1]中国人民解放军联勤保障部队第九七零医院高干一科,山东烟台264001 [2]中国人民解放军71217部队,山东烟台266600

出　　处：《重庆医学》2021年第24期4245-4251,共7页Chongqing medicine

摘　　要：目的应用网络药理学技术分析心宝丸的有效成分及其治疗病态窦房结综合征(SSS)的机制。方法通过TCMSP中药系统药理学数据库和相关文献资料筛选心宝丸的生物活性成分和作用靶点,进而构建心宝丸的药物-有效成分-作用靶点网络。使用Drugbank和Genecards等数据库建立与SSS相关的疾病靶点,并与有效成分靶点匹配获得潜在治疗靶点。对潜在治疗靶点进行GO生物过程、KEGG信号通路分析、蛋白质-蛋白质相互作用(PPI)分析和PPI网络拓扑分析。结果心宝丸中有效化学成分71种,有效成分作用靶点基因271个。在药物-有效成分-靶点网络内前10种重要有效成分中,人参5种,三七2种,洋金花2种,肉桂1种。共收集到1165个SSS相关靶点,其中有31个心宝丸治疗SSS的潜在靶点基因。KEGG富集分析显示,在得出的131条信号通路中,PI3K-Akt、MAPK、cAMP、胆碱能突触、糖尿病并发症中的AGE-RAGE、心肌细胞中的肾上腺素受体、肾素分泌、胰岛素分泌、多巴胺能突触和醛固酮调节钠重吸收信号通路。GO功能富集分析显示,在1220项功能中,生物过程1089项,分子功能74项,细胞定位57项。PPI网络经提取后获得了包含20个节点的核心网络,其中,SCN5A、EDN1、VEGFA、SCN10A、IGF2和VEGFB基因是核心靶点。结论白曼陀罗素、东莨菪碱、山柰酚和桧烯等是心宝丸治疗SSS的潜在有效成分,心宝丸治疗作用的核心靶点主要有SCN5A、EDN1、VEGFA、SCN10A、IGF2和VEGFB基因。Objective To apply the network pharmacology technique to analyze the active ingredients of Xinbao Pills and its mechanism of treatment of pathological sinus node syndrome(SSS).Methods To screen the bioactive components and action targets of Xinbao Pills through TCMSP Traditional Chinese Medicine Systematic Pharmacology Database and other relevant literature,and then construct a drug-active component-target network of Xinbao Pills.Drugbank and Genecards databases were used to identify the SSS-related disease targets and match them with active ingredient targets to obtain potential therapeutic targets.The GO biological processes,KEGG signaling pathways,protein-protein interactions(PPI)and PPI network topologies for potential therapeutic targets were analyzed.Results There were 71 active chemical ingredients and 271 active ingredient target genes in Xinbao Pills.The top ten important active ingredients within the drug-active-ingredient-target network were five ginseng,two panax ginseng,two flos daturae and one cinnamon.A total of 1165 SSS-related targets were collected,of which 31 were potential target genes for the treatment of SSS with Xinbao Pills.The KEGG enrichment analysis revealed that of the total 131 regulatory signaling pathways derived,the PI3K-Akt,MAPK,cAMP,cholinergic synapses,AGE-RAGE in diabetic complications,adrenergic receptors in cardiomyocytes,renin secretion,insulin secretion,dopaminergic synapses and aldosterone could regulate the sodium reabsorption signaling pathways.The GO functional enrichment analysis revealed that of the total 1220 functions,1089 were biological processes,74 were molecular functions and 57 were cellular localizations.The PPI network was extracted to obtain a core network containing 20 nodes,of which the SCN5A,EDN1,VEGFA,SCN10A,IGF2 and VEGFB genes were the core targets.Conclusion The white mandolin,scopolamine,kaempferol and hinokene are potential active ingredients of Xinbao Pills for the treatment of SSS.The core targets of the therapeutic effect of Xinbao Pills are main

关 键 词：心宝丸 病态窦房结综合征 网络药理学 机制 基因 

分 类 号：R54[医药卫生—心血管疾病]