HPLC法测定复方鲜竹沥液中2,6-二甲氧基苯酚的含量  

Determination of 2,6-Dimethoxyphenol in Fufang Xianzhuli Ye by HPLC Method

作　　者：黄东 肖小武 周志强 付辉政 许妍 洪挺 HUANG Dong;XIAO Xiaowu;ZHOU Zhiqiang;FU Huizheng;XU Yan;HONG Ting(Jiangxi Institute for Drug Control,NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine,Jiangxi Province Engineering Research Center of Drug and Medical Device Quality,Nanchang Jiangxi 330029,China)

机构地区：[1]江西省药品检验检测研究院,国家药品监督管理局中成药质量评价重点实验室,江西省药品与医疗器械质量工程技术研究中心,江西南昌330029

出　　处：《药品评价》2021年第22期1356-1359,共4页Drug Evaluation

基　　金：江西省重点研发项目(20203BBG73062);江西省药品监督管理局科研项目(2020JS04)。

摘　　要：目的:测定复方鲜竹沥液中活性成分2,6-二甲氧基苯酚的含量。方法:采用ACE-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸为流动相梯度洗脱;流速为1.0 mL/min;柱温30℃;检测波长201 nm。结果:进样量在0.043~0.43μg之间的2,6-二甲氧基苯酚具有良好的线性关系(r=0.9999),平均回收率为97.3%,RSD为1.7%(n=6)。结论:本研究建立的方法稳定可靠,可控性强,可用于复方鲜竹沥液2,6-二甲氧基苯酚成分的含量测定。Objective:Determination of active component 2,6-dimethoxyphenolin fufang xianzhuli ye.Methods:Using ACE-C18 column(4.6 mm×250 mm,5μm),Acetonitrile-0.1%phosphoric acid as mobile phase with gradient elution.The flow rate was 1.0 mL/min;the column temperature was 30℃;the detection wavelength was 201 nm.Results:The injection amount of 2,6-dimethoxyphenol was 0.043~0.43μg(r=0.9999),showing a good linear relationship,the average recovery was 97.3%,and the RSD was 1.7%(n=6).Conclusion:The established method in this study has high sensitivity,good repeatability and strong specificity,and the method can be used for the determination of the content of 2,6-dimethoxyphenol in fufang xianzhuli ye.

关 键 词：禾本科 复方鲜竹沥液 高效液相色谱法 2 6-二甲氧基苯酚 含量测定 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]