聚邻苯二胺纳米纤维在直接/间接荧光检测及细胞成像中的应用  

Applications of poly o-phenylenediamine nanofiber in direct/indirect fluorescence detection and cell imaging

作　　者：宋金萍[1] 马琦[1] 郭永[1] 焦亚进 SONG Jin-ping;MA Qi;GUO Yong;JIAO Ya-jin(College of Chemistry and Chemical Engineering,Institute of Applied Chemistry,Shanxi Datong University,Datong 037009,China)

机构地区：[1]山西大同大学化学与化工学院,应用化学研究所,山西大同037009

出　　处：《化学研究与应用》2021年第12期2316-2321,共6页Chemical Research and Application

基　　金：山西省自然科学基金项目(201901D111309、201901D111312)资助;大同市工业重点研发计划项目(2019027)资助。

摘　　要：本文以氯化铁氧化的方式制备了聚邻苯二胺(POPD)纳米纤维,并进一步探索了其在直接荧光或间接荧光分析及细胞成像中的应用。实验表明,苦味酸能够显著猝灭POPD的荧光,由此构建的直接荧光传感平台可以用于高灵敏检测苦味酸,检出限低至0.10μmol·L^(-1)。同时鉴于KMnO_(4)良好的氧化性,以其为媒介体构建了间接荧光传感平台,从而实现了对还原性药物对乙酰氨基酚的检测,检出限为1.00μmol·L^(-1)。此外,POPD表现出低细胞毒性和良好的细胞成像能力,可潜在作为生物成像中的荧光探针。In this paper, poly o-phenylenediamine nanofibers were prepared by ferric chloride oxidation method and further explored its applications in direct/indirect fluorescence analysis and cell imaging.Experiments show that picric acid can significantly quench the fluorescence of POPD.Based on this, a direct fluorescence sensing platform is constructed for high sensitive detection of picric acid, and the detection limit is as low as 0.10 μmol·L^(-1).In view of good oxidation property of KMnO_(4),using it as an intermedium, an indirect fluorescence sensing platform is developed to detect the reducing drug acetaminophen with the detection limit of 1.00 μmol·L^(-1).Furthermore, POPD exhibits low cytotoxicity and good cell imaging capability, which can potentially be used as a fluorescent probe in biological imaging.

关 键 词：聚邻苯二胺 纳米纤维 荧光分析 细胞成像 

分 类 号：O657.3[理学—分析化学]