人参皂苷Rbl对小鼠皮肤光老化氧化应激的改善作用  

Ameliorative Effect of Ginsenoside Rbl on Oxidative Stress in Skin Photoaging Mice

作　　者：王倩[1] 万品文[2] 曹丽楠[1] 李雪莉 WANG Qian;WAN Pin-Wen;CAO Li-Nan;LI Xue-Li(Dept.of Dermatology,Nanyang Central Hospital,Nanyang 473000 Henan,China;Dept.of General Surgery,Nanyang Central Hospital,Nanyang 473000 Henan,China)

机构地区：[1]南阳市中心医院皮肤科,河南南阳473000 [2]南阳市中心医院普外科,河南南阳473000

出　　处：《广州中医药大学学报》2021年第11期2479-2484,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：河南省医学科技攻关计划项目(编号:201602206)。

摘　　要：【目的】探讨人参皂苷Rbl对小鼠皮肤光老化的治疗作用及机制。【方法】将成功构建的59只皮肤光老化模型小鼠随机分为模型组,人参皂苷Rbl低、高剂量组各15只,阳性对照组14只,另取15只为正常组。建模成功后第2天,人参皂苷Rb1低、高剂量组分别灌胃人参皂苷Rb1 50、100 mg/kg,阳性对照组灌胃维生素E 30 mg/kg,正常组和模型组灌胃等体积生理盐水,1次/d,连续给药30 d。干预结束,黄嘌呤氧化酶法测定血浆超氧化物歧化酶(SOD)水平,硫代巴比妥酸法测定血浆丙二醛(MDA)水平,酶联免疫吸附分析(ELISA)法测定血浆肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)和白细胞介素1β(IL-1β)水平,苏木素-伊红(HE)染色观察小鼠皮肤组织病理学变化并测量皮肤表皮厚度及纹理评分,蛋白免疫印迹法检测皮肤组织核因子E2相关因子2(Nrf2)、Kelch样环氧氯丙烷相关蛋白1(Keap1)、血红素氧合酶1(HO-1)蛋白相对表达水平。【结果】模型组小鼠表皮层角质层增厚,细胞分层不清,真皮层纤维组织排列紊乱,分布不均,有大量炎性细胞浸润,人参皂苷Rb1低、高剂量组皮肤病理学变化得到改善,但仍可见表皮角质层增厚,真皮层纤维组织断裂、破碎,卷曲扭结,阳性对照组表皮稍有增厚,真皮层可见较完整的纤维组织,少见断裂、破碎,未出现卷曲扭结。与模型组比较,人参皂苷Rb1低、高剂量组和阳性对照组皮肤表皮厚度及纹理评分,MDA、TNF-α、IL-6和IL-1β水平降低,SOD活性及Nrf2、Keap1、HO-1蛋白相对表达水平升高(P <0.05)。【结论】人参皂苷Rbl可降低皮肤光老化小鼠体内氧化应激水平,促进光老化皮肤修复,可能是通过激活Nrf2/Keap1/HO-1信号通路发挥作用。Objective To observe the therapeutic effect and mechanism of ginsenoside Rbl on skin photoaging in mice. Methods Fifty-nine successfully established skin photoaging model mice were randomly divided into model group, ginsenoside Rbl low-dose group(15 cases)and high-dose group(15 cases), positive control group(14 cases)and normal group(15 cases). On the 2 nd day after successful modeling,the ginsenoside Rb1 low-dose and high-dose groups were administrated intragastrically with 50 and 100 mg/kg of ginsenoside Rb1,respectively,the positive control group was administrated intragastrically with 30 mg/kg of vitamin E,while the normal group and model group were intragastric administrated with equal volume of normal saline,the intervention treatment placed once a day for 30 consecutive days. After the intervention, the plasma superoxide dismutase(SOD) level was determined by xanthine oxidase method, the plasma malondialdehyde(MDA) level was determined by thiobarbituric acid method,and the plasma tumor necrosis factor α(TNF-α),interleukin 6(IL-6)and interleukin 1β(IL-1β)levels were determined by enzyme-linked immunosorbent assay(ELISA). Hematoxylineosin(HE)staining was used to observe the histopathological changes of the skin,and skin epidermal thickness and texture score were measured. The relative expression levels of nuclear factor E2-related factor 2(Nrf2),Kelch-like epichlorohydrin-associated protein 1(Keap1)and heme oxygenase 1(HO-1)in skin tissues were detected by Western Blot. Results In the model group,the epidermal stratum corneum of mice was thickened,the cell stratification was unclear,and the dermis fibrous tissue was disorganized and unevenly distributed,with a large number of inflammatory cells infiltrating the skin. The ginsenoside Rb1 low-dose and high-dose groups showed improvement in skin pathology,but thickening of the epidermal stratum corneum,fractured,broken,curling and kinking dermis fibrous tissues were still visible, while the positive control group showed slight thickening of the epidermis an

关 键 词：人参皂苷RB1 光老化 氧化应激 Nrf2/Keap1/HO-1信号通路 小鼠 

分 类 号：R285.5[医药卫生—中药学]