电针干预对LPS诱导帕金森病小鼠黑质NADPH氧化酶表达的影响  

Effect of electroacupuncture on the expression of NADPH oxidase in the substantia nigra of Parkinson disease mice induced by LPS

作　　者：亢恺雯 尹发明 王渊 袁伟 鲁刚 白秋菊 王强 KANG Kaiwen;YIN Faming;WANG Yuan;YUAN Wei;LU Gang;BAI Qiuju;WANG Qiang(The Second Clinical Medical College,Shaanxi University of Chinese Medicine,Shaanxi Province,Xianyang712046,China;Department of Rehabilitation,Qingdao Hospital of Traditional Chinese Medicine,Shandong Province,Qingdao266000,China;College of Acupuncture and Massage,Shaanxi University of Chinese Medicine,Shaanxi Province,Xianyang712046,China)

机构地区：[1]陕西中医药大学第二临床医学院,陕西咸阳712046 [2]青岛市中医医院康复科,山东青岛266000 [3]陕西中医药大学针灸推拿学院,陕西咸阳712046

出　　处：《中国医药导报》2021年第36期19-22,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(81674086);陕西省自然科学基础研究计划项目(2020JM-594)。

摘　　要：目的观察电针干预对脂多糖(LPS)诱导的帕金森病(PD)小鼠黑质还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶亚基p47phox、p22phox表达的影响,探究电针改善PD的作用机制。方法采用随机数字表法,将40只22~25 g清洁级雄性C57BL/6小鼠依次分为空白组、模型组、左旋多巴组、电针组,每组10只。除空白组外,其余各组均经鼻滴注10μl(1 g/L,溶于0.9%氯化钠溶液中)LPS制作PD模型。空白组滴入等量0.9%氯化钠溶液。左旋多巴组腹腔注射左旋多巴注射液0.2 ml(10 mg/kg),电针组针刺“印堂”“迎香”穴(2 Hz/100 Hz,1 mA,20 min),空白组与模型组同等程度抓取。干预结束后对小鼠进行行为学检测,再采用免疫组化法检测黑质细胞中p47phox、p22phox的表达。结果与空白组比较,模型组总进臂次数及正确率均明显减少(P<0.01);与模型组比较,电针组总进臂次数及正确率提高(P<0.05)。与空白组比较,模型组黑质区p47phox、p22phox蛋白阳性细胞数均明显增加(P<0.01);与模型组比较,电针组中黑质区p47phox、p22phox蛋白阳性细胞数均降低(P<0.05)。结论“嗅三针”可抑制PD小鼠的氧化应激,改善其运动症状,延缓PD进程,这可能与其对NADPH氧化酶的抑制有关。Objective To investigate the effect of electroacupuncture on the expression of p47phox and p22phox of reduced nicotinamide adenine dinucleotide phosphate(NADPH)oxidase subunits in the substania nigra of lipopolysaccharide(LPS)induced Parkinson disease(PD)mice,and to explore the mechanism of electroacupuncture in improving PD.Methods Forty male C57BL/6 mice with clean grade of 22-25 g were divided into blank group,model group,Levodopa group,and electroacupuncture group by random number table method,with ten mice in each group.Except blank group,the other groups were given 10μl(1 g/L,dissolved in the 0.9%sodium chloride solution)LPS by nasal drip to make PD model.The blank group was given the same amount of the 0.9%sodium chloride solution.Levodopa group was intraperitoneally injected with 0.2 ml Levodopa Injection(10 mg/kg).Electroacupuncture group acupuncture“yìntáng”“yíngxiāng”point(2 Hz/100 Hz,1 mA,20 min).Blank group and model group were captured in the same degree.After the intervention,the mice were subjected to behavioral testing,and the expression of p47phox and p22phox in substantia nigra cells was detected by immunohistochemical method.Results Compared with blank group,the total number of arm advances and accuracy in model group was decreased significantly(P<0.01).Compared with model group,the total number of arm entry and accuracy of electroacupuncture group was increased(P<0.05).Compared with blank group,the number of p47phox and p22phox protein positive cells in model group was increased significantly(P<0.01).Compared with model group,the number of p47phox and p22phox protein positive cells in the substantia nigra in electroacupuncture group was decreased(P<0.05).Conclusion“Xiu Sanzhen”can inhibit oxidative stress,improve motor symptoms and delay PD process in PD mice,which may be related to the inhibition of NADPH oxidase.

关 键 词：嗅三针 氧化应激 脂多糖 帕金森病 

分 类 号：R245.3[医药卫生—针灸推拿学]