滋阴通络方对去卵巢骨质疏松大鼠骨代谢及骨密度的影响  

Effect of Ziyin Tongluo Decoction on bone metabolism and bone mineral density in ovariectomized osteoporosis rats

作　　者：孙凯 陈荣彬 杨英东 李勇[2] SUN Kai;CHEN Rongbin;YANG Yingdong;LI Yong(The Second Clinical Medical College,Guangzhou University of Chinese Medicine,Guangdong Province,Guangzhou510405,China;the Third Branch of Department of Orthopedics,Zhuhai Hospital,Traditional Chinese Medical Hospital of Guangdong Province,Guangdong Province,Zhuhai519000,China)

机构地区：[1]广州中医药大学第二临床医学院,广东广州510405 [2]广东省中医院珠海医院骨三科,广东珠海519000

出　　处：《中国医药导报》2021年第34期26-29,38,共5页China Medical Herald

基　　金：广东省中医药管理局基金项目(20194010)。

摘　　要：目的探讨滋阴通络方对去卵巢骨质疏松大鼠骨代谢及骨密度的影响。方法取50只SD雌性大鼠建立去卵巢大鼠模型,另取10只SD大鼠设置为假手术组。将建模成功的大鼠按照随机数字表法分为模型组,西药组[阿仑膦酸钠(10.51 mg/kg)与阿法骨化醇(0.075μg/kg)],滋阴通络方低(1.34 g/kg)、中(2.68 g/kg)、高(4.02 g/kg)剂量组,每组10只,对其进行灌胃治疗。治疗12周后,测定各组大鼠骨密度(BMD)、骨微结构及血清中Ⅰ前胶原氨基端原肽(PⅠNP)、Ⅰ型胶原氨基末端肽(NTX)和Ⅰ型胶原C末端肽(CTX-Ⅰ)含量。结果与假手术组比较,模型组BMD、骨体积分数、骨小梁数量、骨小梁厚度及PⅠNP水平降低,差异有统计学意义(P<0.05);骨小梁分离度、骨小梁模式因子、结构模型指数、NTX及CTX-Ⅰ水平升高,差异有统计学意义(P<0.05);与模型组比较,滋阴通络方各剂量组BMD、骨体积分数、骨小梁数量、骨小梁厚度及PⅠNP水平升高;骨小梁分离度、骨小梁模式因子、结构模型指数、NTX和CTX-Ⅰ水平降低,差异有统计学意义(P<0.05)。滋阴通络方不同剂量组组间比较,差异无统计学意义(P>0.05)。结论滋阴通络方可改善去卵巢大鼠骨代谢、提高骨密度,改善骨显微结构,达到治疗绝经后骨质疏松症的作用。Objective To investigate the effect of Ziyin Tongluo Decoction on bone metabolism and bone mineral density in ovariectomized osteoporosis rats.Methods Fifty SD female rats were selected to establish ovariectomized rat model,and another ten SD rats were selected as the sham operation group.The rats were divided into model group,Western medicine group(Alendronate Sodium[10.51 mg/kg]and Alfacalccitalol[0.075μg/kg]),Ziyin Tongluo Decoction low-dose(1.34 g/kg),medium-dose(2.68 g/kg)and high-dose(4.02 g/kg)groups according to random number table method,with ten rats in each group.They were treated by gavage.After 12 weeks of treatment,bone mineral density(BMD),bone microstructure and serum contents of procollagenⅠN-terminal propeptide(PⅠNP),N-terminal telopeptide of typeⅠcollagen(NTX),and C-telopeptide of typeⅠcollagen(CTX-Ⅰ)were determined.Results Compared with sham operation group,BMD,bone volume fraction,bone trabecular number,bone trabecular thickness,and PⅠNP level were decreased in model group,and the differences were statistically significant(P<0.05).The trabecular separation,trabecular pattern factor,structural model index,NTX,and CTX-Ⅰlevels were increased in model group,and the differences were statistically significant(P<0.05).Compared with model group,BMD,bone volume fraction,bone trabecular number,bone trabecular thickness and PⅠNP level in Ziyin Tongluo Decoction groups were increased;the trabecular separation,trabecular pattern factor,structural model index,NTX,and CTX-Ⅰlevels were decreased,and the differences were statistically significant(P<0.05).There was no significant difference between groups with different doses of Ziyin Tongluo Decoction(P>0.05).Conclusion Ziyin Tongluo Decoction can improve bone metabolism,increase bone density and improve bone microstructure of ovariectomized rats,and achieve the effect of postmenopausal osteoporosis treatment.

关 键 词：滋阴通络方 去卵巢大鼠 绝经后骨质疏松症 骨代谢 骨密度 

分 类 号：R285[医药卫生—中药学]