参草通脉方对慢性心力衰竭大鼠心肌ANP、AngⅡ及肾组织cAMP、PKA及PKC含量的影响  

Effects of Shencao Tongmai Formular on ANP,AngⅡof myocardium and cAMP,PKA,and PKC of nephrogenic tissue of chronic heart failure rats

作　　者：礼海[1] 刘晓蕾 王懿[1] 张艳[2] LI Hai;LIU Xiaolei;WANG Yi;ZHANG Yan(Department of Medical,Shenyang Second Hospital of Traditional Chinese Medicine,Liaoning Province,Shenyang110101,China;Expert Consultation,the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Liaoning Province,Shenyang110032,China)

机构地区：[1]沈阳市第二中医医院医务科,辽宁沈阳110101 [2]辽宁中医药大学附属医院专家门诊,辽宁沈阳110032

出　　处：《中国医药导报》2021年第34期35-38,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(81774157)。

摘　　要：目的通过实验观察分析参草通脉方对慢性心力衰竭(CHF)大鼠心肌心房利尿钠肽(ANP)、血管紧张素Ⅱ(AngⅡ)及肾脏环磷酸腺苷(cAMP)、蛋白激酶A(PKA)、蛋白激酶C(PKC)含量的影响。方法选取SPF级12周龄,体重300~350 g的雄性大鼠60只,随机抽取正常组、假手术组(冠状动脉结扎5 s后松开)各8只。剩余大鼠冠状动脉结扎后进行减食、力竭式游泳28 d构建CHF模型,造模成功后采用随机数字表求余数法将其分为中药组、西药组、模型组,每组8只。中药组给予参草通脉复方生药7.2 g/(kg·d),2次/d;西药组每天第一次给药为福辛普利1.2 mg/kg,第二次为蒸馏水1.5 ml;正常组、模型组、假手术组给予蒸馏水1.5 ml灌胃,2次/d,五组均干预28 d。观察五组心肌ANP、AngⅡ及肾脏cAMP、PKA、PKC含量变化。结果模型组ANP、AngⅡ水平高于正常组、假手术组,中药组ANP、AngⅡ水平低于模型组,差异有统计学意义(P<0.05)。模型组cAMP、PKC水平高于正常组、假手术组,PKA低于正常组、假手术组,中药组cAMP、PKC水平低于模型组,PKA高于模型组,差异有统计学意义(P<0.05)。结论参草通脉方能够有效治疗CHF,改善肾脏功能,减少肾脏失代偿后给CHF治疗带来的不利影响。Objective To observe the effects of Shencao Tongmai Formula on atrial natriuretic peptide(ANP),angiotensinⅡ(AngⅡ)of myocardium and cyclic adenosine monophosphate(cAMP),protein kinase A(PKA),and protein kinase C(PKC)of nephrogenic tissue of chronic heart failure(CHF)rats.Methods Sixty SPF 12-week-old male rats weighing 300-350 g were randomly selected as the normal group and the sham operation group(coronary artery ligation was released after five seconds),with eight rats in each group.After coronary artery ligation,the remaining rats were fed down and exhausted swimming for 28 days to construct CHF model.After successful modeling,the rats were divided into traditional Chinese medicine group,Western medicine group,and model group by random number table remainder method,with eight rats in each group.Traditional Chinese medicine group was given Shencao Tongmai Formular crude drug 7.2g/(kg·d),twice a day;Western medicine group was given Fosinopril 1.2 mg/kg for the first time and distilled water 1.5 ml for the second time everyday;normal group,model group,and sham operation group were given distilled water 1.5 ml intragastric administration,twice a day,intervention for 28 d in all five groups.The changes of ANP,AngⅡof myocardium and cAMP,PKA,and PKC of nephrogenic tissue of five groups were observed.Results The levels of ANP and AngⅡin model group were higher than those of normal group and sham operation group,while the levels of ANP and AngⅡin traditional Chinese medicine group were lower than those in model group,the differences were statistically significant(P<0.05).The levels of cAMP and PKC in model group were higher than those of normal group and sham operation group,and PKA was lower than that of normal group and sham operation group.The levels of cAMP and PKC in traditional Chinese medicine group were lower than that of model group,and PKA was higher than that of model group,with statistical significance(P<0.05).Conclusion Shencao Tongmai Formular is efficient in CHF treatment,renal function improv

关 键 词：慢性心力衰竭 心房利尿钠肽 环磷酸腺苷 蛋白激酶A 

分 类 号：R285.5[医药卫生—中药学]