致猪脑膜炎绿色气球菌的分离鉴定与16S rRNA基因序列遗传进化分析  

Isolation,identification and phylogenetic analysis of 16S rRNA gene sequence of Aerococcus viridans causing swine meningitis

作　　者：蒋增海[1,2,3] 孙浩 谢静静 王紫晗 徐耀辉 何启盖[3] JIANG Zenghai;SUN Hao;XIE Jingjing;WANG Zihan;XU Yaohui;HE Qigai(College of Veterinary Medicine,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;Henan Engineering and Technology Research Center of Veterinary Biotechnology,Zhengzhou 450046,China;State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]河南牧业经济学院动物医药学院,河南郑州450046 [2]河南省猪病防控工程技术研究中心,河南郑州450046 [3]华中农业大学农业微生物国家重点实验室,湖北武汉430070

出　　处：《中国兽医学报》2021年第12期2364-2369,共6页Chinese Journal of Veterinary Science

基　　金：财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-35);河南牧业经济学院预防兽医学重点学科建设基金资助项目(MXK2016102)。

摘　　要：为了确定河南省豫西某规模化猪场仔猪发生脑膜炎死亡的病因,无菌采集病料进行细菌的分离培养,通过染色镜检、生化试验和16S rRNA基因序列同源性分析对分离细菌进行鉴定,采取K-B法对分离菌株进行药敏试验。结果显示,从送检3头病死仔猪的脑部组织,以及其中2头病死仔猪的膝关节液,共分离到5株绿色气球菌,命名为P1、T1、P2、T2和P3。16S rRNA基因序列遗传进化分析表明,分离菌株P1、P2、T2、P3属于同一分支,与分离菌株P3亲缘关系较远。药敏试验结果表明,分离菌株对复方新诺明、四环素、头孢曲松耐药率为100%(5/5),对氧氟沙星、头孢噻肟、红霉素耐药率为80%(4/5),对氯霉素、环丙沙星等敏感率相对较高。结果表明,绿色气球菌可能对猪具有致病性,能够导致猪的脑膜炎和关节炎,对兽医临床常用药物耐药性严重,可能导致治疗失败。In order to determine the etiological agent causing the death of piglets by meningitis from a large-scale pig farm in western region of Henan Province,aseptic samples were collected for bacterial isolation and culture.The isolated bacteria were identified by micrographic observation,biochemical test and homology analysis of 16 S rRNA gene sequence.Drug sensitivity test was performed by K-B method.The results showed that five Aerococcus viridans isolates were obtained from the brain tissues of three dead piglets and the knee joint fluid of two dead piglets,they were named P1,T1,P2,T2 and P3.Phylogenetic analysis of 16 S rRNA gene sequence showed that P1,P2,T2 and P3 belonged to the same branch and were far from P3.The result of drug sensitivity test showed that the drug resistance rate of the isolated strains to sulfamethoxazole,tetracycline and ceftriaxone was 100%(5/5),to ofloxacin,cefotaxime and erythromycin was 80%(4/5),and to chloramphenicol and ciprofloxacin was relatively high.This study indicates that Aerococcus viridans may be pathogenic to pigs,can lead to swine meningitis and arthritis.The Aerococcus viridans isolates has serious resistance to commonly used veterinary drugs,which may result in treatment failure.

关 键 词：绿色气球菌 脑膜炎 分离鉴定 16S rRNA基因 猪 

分 类 号：S852.61[农业科学—基础兽医学] R535[农业科学—兽医学]