E.tenella胞内生殖前期对宿主细胞的损伤作用  

Injury effect of E.tenella in prophase of intracellular reproduction on host cells

作　　者：吕晓玲 赵素芬 郑明学[1] 王运盛 张黎 LYU Xiaoling;ZHAO Sufen;ZHENG Mingxue;WANG Yunsheng;ZHANG Li(College of Veterinary Medicine,Shanxi Agricultural University,Taigu,Shanxi 030801,China;Beijing Zoo,Beijing 100044,China)

机构地区：[1]山西农业大学动物医学学院,山西太谷030801 [2]北京市动物园,北京100044

出　　处：《中国兽医学报》2021年第12期2385-2390,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31972647);山西省青年科技研究资助项目(201901D211373);山西农业大学科技创新资助项目(2020BQ08);山西省优秀博士科研奖励计划资助项目(SXYBKY2019023)。

摘　　要：在柔嫩艾美耳球虫(Eimeria tenella,E.tenella)感染原代鸡胚盲肠上皮细胞模型的基础上,通过细胞形态学观测、透射电镜观察,免疫组化染色、MTT法、乳酸脱氢酶活性检测以确定E.tenella胞内生殖前期是否会损伤宿主细胞。结果显示,E.tenella胞内生殖前期对宿主细胞的形态并无明显影响,但可损伤胞内细胞器,可使细胞骨架发生重排;对细胞活性无明显影响且细胞膜无明显损伤。结果表明,E.tenella胞内生殖前期对宿主细胞有一定的损伤,但并不影响细胞活性,该结果为深入探究E.tenella致机体损伤机制提供研究基础。Eimeria tenella(E.tenella)is a typical intracellular parasitic protozoan,which can seriously damage the cecum.In order to explore whether E.tenella could damage the host cells during the prophase of intracellular reproduction,the primary chicken embryo cecal epithelial cells model of E.tenella infection was used.Cell morphology observation,transmission electron microscope observation,immunohistochemical staining,MTT,lactic dehydrogenase activity detection were performed to determine whether E.tenella would damage the host cells during the prophase of intracellular reproduction.The results showed that E.tenella in the prophase of intracellular reproduction had no significant effect on the morphology of host cells,but it could damage the organelles and rearrange the cytoskeleton.It had no significant effect on cell viability and cell membranes.These results suggest that E.tenella has a certain degree of damage to host cells during the prophase of intracellular reproduction.This study provides a research basis for exploring the pathogenic mechanism of E.tenella.

关 键 词：柔嫩艾美耳球虫 原代鸡胚盲肠上皮细胞 细胞损伤 

分 类 号：S852.723[农业科学—基础兽医学]