负载活性组分的核壳结构纤维膜的制备及性能  

Preparation and performance of core-shell structure fiber membranes loaded with active components

作　　者：郝中乾 王曼婷 张成桂 乐园[1,3] HAO Zhongqian;WANG Manting;ZHANG Chenggui;LE Yuan(State Key Laboratory of Organic-Inorganic Composites,Beijing University of Chemical Technology,Beijing 100029,China;Research Center of the Ministry of Education for High Gravity Engineering and Technology,Beijing University of Chemical Technology,Beijing 100029,China;Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D,Dali University,Dali 671000,Yunnan,China)

机构地区：[1]北京化工大学有机-无机复合材料国家重点实验室,北京100029 [2]大理大学云南省昆虫生物医药研发重点实验室,北京100029 [3]北京化工大学教育部超重力工程研究中心,云南大理671000

出　　处：《精细化工》2021年第12期2465-2470,2545,共7页Fine Chemicals

摘　　要：采用同轴静电纺丝技术制备了用于伤口修复的核壳结构纳米纤维膜,将蛛丝蛋白(Ss)和美洲大蠊提取物(PAE)分别负载于纳米纤维的壳层与核层。采用SEM和TEM对纳米纤维膜的形貌进行了表征,结果显示,纤维具有明显的核壳结构,且随着Ss含量的增加,纤维直径从350 nm降至280 nm,核层直径由120 nm升至140 nm,壳层厚度由115 nm降至70 nm;FTIR结果证明Ss已成功负载到纤维膜中。纤维膜的物理性能测定实验表明,制备的纳米纤维膜拉伸强度可达4.3 MPa,溶胀率可达150%,水蒸气透过率可达1834 g/(m^(2)·24 h),水接触角减小到32.7°。药物释放实验结果显示,7 d内药物释放可达77%;考察了纳米纤维膜的生物相容性,相较于未负载Ss的纳米纤维膜,Ss含量为20%的纤维膜的细胞增殖率提高了25%。Nanofiber membranes with core-shell structure for wound repair were prepared by coaxial electrospinning technique. Spider silk protein(Ss) and Periplaneta americana Linnaeus extract(PAE) were respectively loaded in the shell and core layer of nanofiber. The morphology of nanofiber membrane was characterized by SEM and TEM. SEM and TEM results revealed that the fiber had an obvious core-shell structure. With the increase of Ss content, the diameter of nanofiber decreased from 350 nm to 280 nm, the diameter of core increased from 120 nm to 140 nm, and the thickness of shell decreased from 115 nm to 70 nm. FTIR results verified the successful loading of Ss into the nanofiber membrane. The physical properties of nanofiber membrane were determined and the results indicated that the tensile strength could reach 4.31 MPa, the swelling rate could reach 150%, the water vapor permeability rate could reach 1834 g/(m^(2)·24 h)and the water contact angle could be reduced to 32.7°. The results of drug release experiment showed that drug release could reach 77% within 7 d. The biocompatibility of nanofiber membrane was also investigated. Compared with that of unloaded with Ss, cell proliferation of nanofiber membrane with 20%content Ss was increased by 25%.

关 键 词：同轴静电纺丝 蛛丝蛋白 美洲大蠊提取物 核壳结构 药物缓释 功能材料 

分 类 号：TQ340.64[化学工程—化纤工业] R318.08[医药卫生—生物医学工程]