茎瘤固氮根瘤菌ORS571中motA基因的功能分析  

Functional analysis of motA gene in Azorhizobium caulinodans ORS571

作　　者：孙丽 王丹丹 殷志秋 油勇强 刘润进 解志红 Li Sun;Dandan Wang;Zhiqiu Yin;Yongqiang You;Runjin Liu;Zhihong Xie(College of Resourses and Environment,Shandong Agricultural University,Tai’an 271000,Shandong Province,China;Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,Shandong Province,China;College of Plant Health&Medicine,Qingdao Agricultural University,Qingdao 266000,Shandong Province,China)

机构地区：[1]山东农业大学资源与环境学院,山东泰安271000 [2]中国科学院烟台海岸带研究所,山东烟台264003 [3]青岛农业大学植物医学学院,山东青岛266000

出　　处：《微生物学报》2021年第12期4048-4058,共11页Acta Microbiologica Sinica

基　　金：国家自然科学基金(31870020);NSFC-山东省联合基金重点支持项目(U1806206)。

摘　　要：【目的】MotA是细菌的鞭毛马达蛋白,是跨膜质子通道的重要组成结构之一,在调控鞭毛运动中具有至关重要的作用。本研究探究了Azorhizobium caulinodans ORS571中鞭毛马达基因motA对菌株表型和植物互作的影响。【方法】通过同源重组原理和三亲接合转移方法构建突变菌株ΔmotA,测定野生型与突变体在菌体生长、运动、固氮、胞外多糖合成、生物膜形成及根系定殖能力的差异。【结果】与野生型相比,突变体菌体生长没有明显差异,但其运动能力完全丧失,固氮、胞外多糖合成、生物膜形成及根系定殖能力减弱。【结论】MotA鞭毛马达蛋白对A.caulinodans ORS571的运动、固氮、胞外多糖合成、生物膜形成及根系定殖能力均有调控作用。[Objective]MotA,an important flagellar motor protein regulating flagellar motility,is one of components of transmembrane proton channel.In this study,we explored the role of motA in Azorhizobium caulinodans ORS571 in the free-living and symbiotic states.[Methods]ΔmotA mutant was constructed by homologous recombination and tri-parental conjugation.We tested the characterizations of the growth state,swimming motility,nitrogen fixation,exopolysaccharides(EPS)production,biofilm formation,and host colonization between wild-type andΔmotA mutant.[Results]ΔmotA mutant showed defective cell motility ability but the growth property was not impaired,and its nitrogen fixation,EPS production,biofilm formation,and host colonization were lower than that of the wild-type.[Conclusion]In A.caulinodans ORS571,MotA plays role in motility,nitrogen fixation,EPS production,biofilm formation,and root colonization.

关 键 词：茎瘤固氮根瘤菌ORS571 鞭毛马达蛋白 motA 

分 类 号：Q78[生物学—分子生物学]