秦皮乙素调控miR-486-5p表达抑制高糖诱导的肾小管上皮细胞炎症损伤的机制研究  

Mechanism of esculetin inhibiting high-glucose induced inflammatory injury of renal tubular epithelial cells by regulating expression of miR-486-5p

作　　者：成涛 王桂林[1] 张秀容[1] 张涛[1] 唐前容 CHENG Tao;WANG Guilin;ZHANG Xiurong;ZHANG Tao;TANG Qianrong(Department of Emergency,Yibin City Second People′s Hospital in Sichuan Province,Yibin,Sichuan,644000)

机构地区：[1]四川省宜宾市第二人民医院急诊科,四川宜宾644000

出　　处：《实用临床医药杂志》2021年第19期83-87,共5页Journal of Clinical Medicine in Practice

摘　　要：目的探讨秦皮乙素(Esc)对高糖诱导的肾小管上皮细胞炎症损伤的影响及分子机制。方法肾小管上皮细胞HK-2随机分为对照(Con)组,高糖(HG)组,Esc低、中、高浓度(HG+Esc-L、M、H)组,HG+anti-miR-NC组,HG+anti-miR-486-5p组,HG+Esc+miR-NC组以及HG+Esc+miR-486-5p组。采用酶联免疫吸附法(ELISA)检测白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平;采用流式细胞术检测细胞凋亡率;采用实时荧光定量聚合酶链式反应(RT-qPCR)检测miR-486-5p表达水平。结果Esc低、中、高浓度处理后,高糖诱导的HK-2细胞中IL-6、TNF-α水平及miR-486-5p表达水平逐渐降低,凋亡率逐渐降低,差异有统计学意义(P<0.05)。抑制miR-486-5p表达后,高糖诱导的HK-2细胞中IL-6、TNF-α水平及细胞的凋亡率降低,差异有统计学意义(P<0.05)。过表达miR-486-5p和Esc同时处理后,IL-6、TNF-α水平及细胞的凋亡率升高,差异有统计学意义(P<0.05)。结论Esc通过下调miR-486-5p表达抑制高糖诱导的肾小管上皮细胞炎症损伤。Objective To explore the effect of esculetin(Esc)on the inflammatory injury of renal tubular epithelial cells induced by high glucose and its molecular mechanism.Methods Renal tubular epithelial cells HK-2 were randomly divided into control(Con)group,high glucose(HG)group,low,medium and high concentration of Esc(HG+Esc-L,M,H)groups,HG+anti-miR-NC group,HG+anti-miR-486-5p group,HG+Esc+miR-NC group and HG+Esc+miR-486-5p group.Enzyme-linked immunosorbent assay(ELISA)was used to detect interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)levels.Flow cytometry was used to detect cell apoptosis rate.Real-time fluorescent quantitative polymerase chain reaction(RT-qPCR)was used to detect miR-486-5p expression level.Results After treatment with low,medium and high concentrations of Esc,the levels of IL-6 and TNF-αand miR-486-5p expression in HK-2 cells induced by high glucose were gradually decreased,while the apoptosis rate was gradually decreased,and there were significant differences between groups(P<0.05).After inhibiting the expression of miR-486-5p,the levels of IL-6,TNF-αand the apoptosis rate in HK-2 cells induced by high glucose were decreased(P<0.05).After simultaneous treatments with over-expression of miR-486-5p and Esc,IL-6 and TNF-αlevels and cell apoptosis rate were increased(P<0.05).Conclusion Esc inhibits the inflammatory injury of renal tubular epithelial cells induced by high glucose by down-regulating the expression of miR-486-5p.

关 键 词：秦皮乙素 miR-486-5p 肾小管上皮细胞 凋亡 炎症反应 

分 类 号：R587.2[医药卫生—内分泌] R285.6[医药卫生—内科学]