TEAseq-based identification of 35,696 Dissociation insertional mutations facilitates functional genomic studies in maize  

作　　者：Mingjie Lyu Huafeng Liu Joram Kiriga Waititu Ying Sun Huan Wang Junjie Fu Yanhui Chen Jun Liu Lixia Ku Xiliu Cheng 

机构地区：[1]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]College of Agronomy,Collaborative Innovation Center of Henan Grain Crops and National Key Laboratory of Wheat and Maize Crop Science,Henan Agricultural University,Zhengzhou,Henan 450002,China [3]Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《Journal of Genetics and Genomics》2021年第11期961-971,共11页遗传学报（英文版）

基　　金：the Ministry of Science and Technology of China(2016YFD0101000 and 2016YFD0101001);the Natural Science Foundation of China(31901595).

摘　　要：In plants,transposable element(TE)-triggered mutants are important resources for functional genomic studies.However,conventional approaches for genome-wide identification of TE insertion sites are costly and laborious.This study developed a novel,rapid,and high-throughput TE insertion site identification workflow based on next-generation sequencing and named it Transposable Element Amplicon Sequencing(TEAseq).Using TEAseq,we systemically profiled the Dissociation(Ds)insertion sites in 1606 independent Ds insertional mutants in advanced backcross generation using K17 as background.The Ac-containing individuals were excluded for getting rid of the potential somatic insertions.We characterized 35,696 germinal Ds insertions tagging 10,323 genes,representing approximately 23.3%of the total genes in the maize genome.The insertion sites were presented in chromosomal hotspots around the ancestral Ds loci,and insertions occurred preferentially in gene body regions.Furthermore,we mapped a loss-of-function AGL2 gene using bulked segregant RNA-sequencing assay and proved that AGL2 is essential for seed development.We additionally established an open-access database named MEILAM for easy access to Ds insertional mutations.Overall,our results have provided an efficient workflow for TE insertion identification and rich sequence-indexed mutant resources for maize functional genomic studies.

关 键 词：MAIZE Zea mays Insertion identification Ds transposon Mutant library Next-generation sequencing Functional genomics 

分 类 号：S513[农业科学—作物学]