兴义维蚋幼虫扭动摄食方式的神经-肌肉结构基础  

The structure basis of neuromuscular junction of the pattern of twisting and feeding of Simulium xingyiense larvae

作　　者：张洪卫 高晗 寻慧 杨明 ZHANG Hong-Wei;GAO Han;XUN Hui;YANG Ming(School of Basic Medicine,Department of Biology and Key Laboratory of Medical Entomology,Guizhou Medical University,Guiyang 550025,China)

机构地区：[1]贵州医科大学基础医学院,生物学系暨医学昆虫重点实验室,贵阳550025

出　　处：《环境昆虫学报》2021年第6期1515-1521,共7页Journal of Environmental Entomology

基　　金：国家自然科学基金(31860324)。

摘　　要：蚋类幼虫为河流清洁者,可作为水污染的指标。本文用抗辣根过氧化物酶抗体(anti-HRP)和鬼笔环肽(phalloidin),通过免疫荧光显示兴义维蚋Simulium xingyiense幼虫腹神经索及外周神经与肌肉连接结构。结果表明幼虫腹神经索由11对神经节及连接的神经索组成。每对体神经节由两团神经胞体聚合而成,细胞核分布呈无头无尾的Φ形。在1~5腹神经节两侧,纵神经连接横神经呈现“梯形结构”;虫体腹部肌肉分横肌、纵肌和斜肌3类肌群。神经节中部发出两对侧神经入腹肌,再继续分支,其神经末梢呈链珠状突触结构连接肌肉,增加神经与肌肉的接触面积。这种神经-肌肉的连接方式可能与兴义维蚋幼虫扭动摄食行为有关。本研究初步阐述了兴义维蚋幼虫腹神经索及腹部神经与肌肉连接的细微结构基础。Simulium larvae are a cleaner and a pollution indicator of the water quality in natural river system.In this study,the anti-HRP antibody and phalloidin were used to mark the peripheral nerves of ventral nerve cord and muscles and showed the structure of abdominal neuromuscular junctions in the larvae of Simulium xingyiense by immunofluorescence.The ventral nerve cord consisted of eleven pairs of ganglia in larvae.Each pair of ganglia was formed by two coalesced masses of neurosomes,and the distribution pattern of nucleus was a headless and tailless Φ shape.The lateral nerves and longitudinal nerves were connected on both sides of AG1~AG5 and the connection structure looked like a trapezoidal shape.The abdominal muscles were divided into three types,namely transverse,longitudinal,and oblique.The transverse nerve extended into the abdominal longitudinal muscles on both sides of ganglion,and the nerve endings separates from it were formed a bead-like bulging terminal boutons structure.The structure basis of neuromuscular junctions might be related to the pattern of twisting and feeding of S.xingyiense larvae.In this paper,we described the fine structural basis of the ventral nerve cord and abdominal neuromuscular junctions in the larvae of S.xingyiense.

关 键 词：兴义维蚋 腹神经索 anti-HRP抗体 PHALLOIDIN 神经-肌肉连接 

分 类 号：Q963[生物学—昆虫学] S489[农业科学—农药学]