三种中兽药产品中黄柏薄层色谱无苯展开系统研究  

The Benzene-free Spread Solvent Study about the Thin Layer Chromatography of Phelendron chinensis in Three Chinese Veterinary Medicine Products

作　　者：安鹏丽[1] 陈静[1] 李金超 冯亚文 郭莉 张娜[1] 杨彦超 李楼 武侠均 AN Peng-li;CHEN Jing;LI Jin-chao;FENG Ya-wen;GUO Li;ZHANG Na;YANG Yan-chao;LI Lou;WU Xia-jun(Baoding Jizhong Pharmaceutical Co. Ltd,Baoding 071500, China;Hebei Provincial Institute of Supervision for Veterinary Drug,Shijiazhuang 050035, China;Tangshan Food and Drug Comprehensive Testing Center, Tangshan, Hebei 063000, China)

机构地区：[1]保定冀中药业有限公司,保定071500 [2]河北省兽药监察所,石家庄050035 [3]唐山市食品药品综合检验检测中心,河北唐山063000

出　　处：《中国兽药杂志》2021年第12期25-30,共6页Chinese Journal of Veterinary Drug

摘　　要：为了降低在薄层色谱分析中苯对人体的危害及对环境的污染,研制可替代黄柏薄层色谱鉴别展开系统中的苯试剂,本研究对常用中兽药三黄散、公英青蓝合剂、公英青蓝颗粒进行了展开剂的系列优化试验。结果表明,以正己烷-三氯甲烷-丁酮-乙酸乙酯-甲酸-水(3∶9∶6∶3∶4∶0.5)为展开剂,能够对以上三种药物达到较好的分离效果,薄层色谱图中主斑点盐酸小檗碱与其上下斑点分离度好,外形清晰圆整,为进一步完善三种药物的质量标准提供了试验依据。In order to reduce the harm of benzene to human body and the pollution to the environment,replace the use of benzene reagents in the development system of Phellodendron chinense thin layer chromatography identification,our laboratory had optimized the development agent of commonly used veterinary drugs--Sanhuang San,Gongying Qinglan Keli,Gongying Qinglan Heji.The results indicated that it can achieve better separation effect for all the three drugs with n-hexane-trichloroform-butanone-ethyl acetate-formic acid-water(3∶9∶6∶3∶4∶0.5)as the development systems.The main spots of berberine hydrochloride are effective separated with other spots in the TLC figure,and all the spots visible are clear with round appearance.The results may provide experimental basis for further improving the quality standars of the three drugs.

关 键 词：黄柏 盐酸小檗碱 薄层色谱 苯 优化 

分 类 号：S859.79[农业科学—临床兽医学]