维生素D_(3)软胶囊中抗氧化剂二丁基羟基甲苯的含量测定  

Determination of Dibutyl Hydroxy Toluene in Vitamin D_(3) Soft Capsules

作　　者：王秀杰[1,2] 张静 赵功宝[1] 付宝慧[1] WANG Xiu-jie;ZHANG Jing;ZHAO Gong-bao;FU Bao-hui(Xuzhou Pharmaceutical Vocational School,Xuzhou Jiangsu 221000,China;Xuzhou Maternal arid Child Health Hospital,Xuzhou Jiangsu 221000,China)

机构地区：[1]徐州医药高等职业学校,江苏徐州221000 [2]徐州市妇幼保健院,江苏徐州221000

出　　处：《抗感染药学》2021年第10期1417-1420,共4页Anti-infection Pharmacy

基　　金：江苏省药学会基金项目(编号:H202046)。

摘　　要：目的:建立维生素D_(3)软胶囊中抗氧化剂二丁基羟基甲苯(dibutyl hydroxy toluene,BHT)含量的高效液相色谱测定方法,分析其可行性。方法:采用Diamonsil C;柱(5μm,250 mm×4.6 mm)为色谱柱,以BHT对照品及维生素D_(3)软胶囊为测定对象,依次制备对照品溶液、供试品溶液与空白溶液,考察其在35℃、278 nm、1.0 L/min、5μm条件下检测的精密度、重复性、回收率、稳定性试验和样品的含量的可行性。结果:得线性方程A=10 066.89ρ-1 030.5(r2=0.999 9),在浓度为5.46~16.30μg/m L范围内,ρ与A呈良好线性关系,表明其精密度、重复性、回收率及稳定性试验结果良好,测定的样本含量符合要求。结论:建立的维生素D_(3)软胶囊中BHT含量的HPLC,具有较好的准确性与便捷性,可用于维生素D_(3)软胶囊中BHT含量测定。Objective:To establish a HPLC method for the determination of antioxidant dibutyl hydroxytoluene(BHT) in vitamin D_(3)soft capsules and analyze its feasibility.Methods:The column was Diamonsil C;column (5μm,250 mm×4.6 mm),and the BHT control substance and vitamin D_(3)soft capsule were determined.The precision,repeat‐ability,recovery,stability test and the feasibility of reference solution,test solution and blank solution were studied at35℃,278 nm,1.0 L/min and 5μm.Results:The linear equation A=10 066.89ρ-1 030.5 (r2=0.999 9),ρand A in the concentration range was between 5.46 and 16.30μg/m L;a good linear relationship,indicating that the precision,re‐peatability,recovery and stability test results are good,the determination of sample content meets the requirements.Conclusion:The established HPLC method has good accuracy and convenience,and can be used for the determination of BHT in vitamin D_(3)soft capsules.

关 键 词：维生素D_(3)软胶囊 抗氧化剂 二丁基羟基甲苯 高效液相色谱法 含量测定 

分 类 号：TQ460.72[医药卫生—药物分析学]