人参皂苷Rh2诱导肝癌细胞HepG2自噬与凋亡的分子机制研究  

Study on Molecular Mechanism of Ginsenoside Rh2 Inducing Autophagy and Apoptosis in Hepatocarcinoma Cells HepG2

作　　者：高涵[1] 吴琦[1] 陈宏月 郭红艳[1] 周涛 刘哲丞[1] GAO Han;WU Qi;CHEN Hongyue;GUO Hongyan;ZHOU Tao;LIU Zhecheng(Department of Biochemistry and Molecular Biology,Qiqihar Medical College,Qiqihar,Heilongjiang Province,161006 China;General Surgery,the First Affiliated Hospital of Qiqihar Medical College,Qiqihar,Heilongjiang Province,161041 China)

机构地区：[1]齐齐哈尔医学院生物化学与分子生物学教研室,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院附属第一医院普通外科,黑龙江齐齐哈尔161041

出　　处：《系统医学》2021年第22期37-40,共4页Systems Medicine

基　　金：黑龙江省教育厅基本科研业务费基础研究项目(2017-KYYWF-0732)。

摘　　要：目的研究人参皂苷Rh2与肝癌细胞自噬的相关性,并探讨作用机制。方法免疫荧光法检测半胱氨酸蛋白酶-8/9(caspase-8/9)活性及自噬相关基因Beclin-1和LC3的表达。结果免疫荧光实验表明,20μg/mL人参皂苷Rh2作用肝癌HepG2细胞3 h,caspase-8活性和Beclin-1表达水平明显升高;caspase-9活性和LC3表达无显著变化。结论人参皂苷Rh2可以调节膜受体途径caspase-8和自噬相关基因Beclin-1的表达,发挥促进肿瘤细胞凋亡的生物学作用。Objective To study the correlation between ginsenoside Rh2 and autophagy of liver cancer cells,and to explore the mechanism of action.Methods Immunofluorescence method was used to detect the activity of caspase-8/9(caspase-8/9)and the expression of autophagy-related genes Beclin-1 and LC3.Results Immunofluorescence experiments showed that when 20μg/mL ginsenoside Rh2 acted on HepG2 cells for 3 h,caspase-8 activity and Beclin-1 expression levels were significantly increased;caspase-9 activity and LC3 expression did not change significantly.Conclusion Ginsenoside Rh2 can regulate the expression of membrane receptor pathway caspase-8 and autophagy-related gene Beclin-1,and play a biological role in promoting tumor cell apoptosis.

关 键 词：人参皂苷RH2 肝癌细胞 caspase-8/9 BECLIN-1 LC3 

分 类 号：R285[医药卫生—中药学]