润滑障碍中的lncRNA-mRNA共表达网络的构建及功能预测  

Construction and function prediction of lncRNA-mRNA co-expression network in lubrication disorder

作　　者：刘天娇 张晶晶[2] 马洁桦[3] 潘连军[4] 阮红杰[5] LIU Tianjiao;ZHANG Jingjing;MA Jiehua;PAN Lianjun;RUAN Hongjie(Department of Women’s Health Care,the Affiliated Obstetrics and Gynaecology Hospital of Nanjing Medical University,Nanjing 210004,China;Obstetrics Department,the Affiliated Obstetrics and Gynaecology Hospital of Nanjing Medical University,Nanjing 210004,China;Medical Research Center,the Affiliated Obstetrics and Gynaecology Hospital of Nanjing Medical University,Nanjing 210004,China;Department of Urology,the Affiliated Obstetrics and Gynaecology Hospital of Nanjing Medical University,Nanjing 210004,China;Department of Emergency,the Affiliated Obstetrics and Gynaecology Hospital of Nanjing Medical University,Nanjing 210004,China)

机构地区：[1]南京医科大学附属妇产医院妇女保健科,江苏南京210004 [2]南京医科大学附属妇产医院产科,江苏南京210004 [3]南京医科大学附属妇产医院医学研究中心,江苏南京210004 [4]南京医科大学附属妇产医院泌尿外科,江苏南京210004 [5]南京医科大学附属妇产医院急诊医学科,江苏南京210004

出　　处：《南京医科大学学报（自然科学版）》2021年第11期1585-1591,共7页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金项目(81771572)。

摘　　要：目的:通过构建阴道润滑障碍(lubrication disorder,LD)女性阴道上皮组织差异表达lncRNA-mRNA共表达网络,探索LD患者的潜在发病机制。方法:利用以|Log_(2)FC|≥2且校正后P值<0.05,鉴别LD和正常对照组之间的长链非编码RNA(long non-coding RNA,LncRNA)以及mRNA的表达谱,利用Cytoscape软件构建差异表达lncRNA及差异表达mRNA网络,采用Gene Ontology(GO)和KEGG Pathway分析共表达网络中mRNA的生物学功能。结果:通过下一代测序技术,根据|Log_(2)FC|≥2且校正后P值<0.05标准筛选出LD组和正常对照组中共计499条上调表达的lncRNA、337条下调表达的lncRNA,以及1582条上调表达mRNA、633条下调表达的mRNA。随后,通过其中100个lncRNA与311个mRNA构建了基于差异表达的lncRNA和mRNA的共表达网络。最后,共表达网络的功能富集分析表明mRNA主要与心肌相关的疾病和功能有关。结论:LD的发病机制可能与血液循环功能障碍、局部肌肉功能障碍以及cGMP通路等有关,需要相关研究来进一步证实。Objective:This study aims to use bioinformatics methods to screen the differentially long non-coding RNA(LncRNA)in vaginal epithelial tissues of women with vaginal lubrication disorder(LD),and to construct patients with vaginal lubrication disorder based on the hypothesis of lncRNA-mRNA co-expression network to further explore the potential pathogenesis of patients with vaginal LD,in order to provide new ideas for the diagnosis and treatment of patients with vaginal LD.Methods:Using|Log_(2)FC|≥2 and correct P ralue<0.05,the expression profile of long-chain non-coding RNA and mRNA between LD group and normal control group were identified.Using Cytoscape software,a lncRNA-mRNA network of candidate lncRNAs was constructed.Using Gene Ontology(GO)and KEGG Pathway,the biological functions of mRNAs in co-expression network were analyzed.Results:A total of 499 up-regulated lncRNAs,337 down-regulated lncRNAs,and 1582 up-regulated mRNAs,633 were selected in the LD group and the normal control group based on the next-generation sequencing technology according to the criteria of|Log_(2)FC|≥2 and correct P ralue<0.05.Subsequently,a lncRNA-mRNA co-expression network was constructed based on differentially expressed lncRNA and mRNA through100 lncRNAs and 311 mRNAs.Finally,the functional enrichment analysis of the lncRNA-mRNA network showed that m RNA was mainly related to myocardial-related diseases and functions.Conclusion:The pathogenesis of LD may be related to blood circulation dysfunction,local muscle dysfunction,and cGMP pathway,etc.This requires further studies at the cellular level,animal level and even human level to confirm.

关 键 词：阴道润滑障碍 长链非编码RNA 共表达网络 

分 类 号：R711.73[医药卫生—妇产科学]