5种活血化瘀类中药注射液对大鼠局灶性脑缺血损伤的保护作用研究  

Protective effect of five traditional Chinese medicine injections for activating blood and removing blood stasis on focal cerebral ischemia injury in rats

作　　者：王志强[1] 李斌[1] 穆珊珊[1] 张秀清[1] 王洋[1] 庄伟 王正杰 WANG Zhiqiang;LI Bin;MU Shanshan;ZHANG Xiuqing;WANG Yang;ZHUANG Wei;WANG Zhengjie(Tangshan People's Hospital,Tangshan 063001,China)

机构地区：[1]唐山市人民医院,河北唐山063001

出　　处：《药物评价研究》2021年第11期2356-2364,共9页Drug Evaluation Research

摘　　要：目的研究5种活血化瘀类中药注射剂对大鼠局灶性脑缺血损伤的保护作用。方法制备大鼠暂时性左侧中动脉栓塞(middle cerebral artery occlusion,tMCAO)模型,造模成功后实验动物随机分成7组,分别为模型组、注射用丹参多酚酸(11.71 mg/kg)组、注射用丹参多酚酸盐(17.86 mg/kg)组、银杏内酯注射液(0.91 mL/kg)组、银杏二萜内酯葡胺注射液(0.45mL/kg)组、注射用血塞通(冻干)(18.02 mg/kg)组和丁苯酞氯化钠注射液(阳性药,9 mL/kg)组。假手术组同法造模,不栓塞大脑中动脉。各组大鼠尾iv给药14 d后,评估神经功能评分;干湿比质量法测定脑含水量;TTC染色法评价脑梗死面积;伊文思蓝法测定血脑屏障通透性;酶联免疫吸附试剂盒检测组织匀浆中白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、γ-干扰素(IFN-γ)、肿瘤坏死因子-α(TNF-α)水平;生化分析法测定丙二醛(MDA)、超氧化物歧化酶(SOD)活性;HE染色法检测组织病理学变化;末端标记法(TUNEL)观察神经细胞及神经元凋亡情况。结果给药后,与模型组比较,各给药组神经功能评分均降低,其中注射用丹参多酚酸组和注射用丹参多酚酸盐组差异显著(P<0.01);各给药组脑含水量均不同程度的降低,除注射用血塞通(冻干)和丁苯酞氯化钠注射液组外,其他各给药组均差异显著(P<0.01);各给药组脑梗死面积均显著下降,差异有统计学意义(P<0.01);各给药组给药后伊文思蓝渗出量均有不同程度的降低,其中注射用丹参多酚酸、注射用丹参多酚酸盐、注射用血塞通(冻干)和丁苯酞氯化钠注射液组差异显著(P<0.05、0.01);各给药组IFN-γ、IL-1β、IL-6和TNF-α含量均显著降低,差异均有统计学意义(P<0.05、0.01);各给药组均能不同程度抑制SOD的降低,其中注射用丹参多酚酸和注射用银杏内酯注射液组差异有统计学意义(P<0.01);各给药组均能不同程度抑制MDA的升高,其中注射用丹参多酚酸、�Objective To study the protective effect of five traditional Chinese medicine injections for promoting blood circulation and removing blood stasis on focal cerebral ischemic injury in rats.Method Preparation the left side of the artery embolism in rats(Middle cerebral artery occlusion,MCAO).After successful modeling,the experimental animals were randomly divided into eight groups:model group,Salvianolic Acids for Injection(11.71 mg/kg)group,Salvianolate for Injection(17.86 mg/kg)group,Ginkgolide Injection(0.91 mL/kg)group,Ginkgo Diterpene Lactone Meglumine Injection(0.45 mL/kg)group,Xuesetong for Injection(freeze-dried,18.02 mg/kg)group,and Butylphthalide Sodium Chloride for Injection(positive drug,9 mL/kg)group.After 14 days of caudal vein administration,neurological function score was evaluated in each group.The moisture content of brain was determined by dry and wet specific gravity method.The area of cerebral infarction was evaluated by TTC staining.The blood-brain barrier permeability was determined by Evensland method.The levels of IL-6,IL-1,IFN-and TNF-αin tissue homogenizer were detected by ELISA kit.The activity of MDA and SOD was measured by biochemical analysis.HE staining was used to detect histopathological changes.Apoptosis of neuronal cells and neurons was observed by Tunel.Results After administration,compared with the model group,the neurological function scores of each administration group decreased,and there was significant difference between Salvianolic Acids for Injection and Salvianolate for injection group(P<0.01).Except Xuesetong for Injection(freezedried)group and Butylphthalide Sodium Chloride for Injection group,the brain water content of each treatment group decreased significantly(P<0.01).The area of cerebral infarction in each treatment group decreased significantly(P<0.01).Evans blue exudation decreased in different degrees in each treatment group,and there were significant differences in Salvianolic Acids for Injection group,Salvianolate for Injection group,Xuesetong for Injection

关 键 词：中药注射剂 注射用丹参多酚酸 注射用丹参多酚酸盐 银杏内酯注射液 银杏二萜内酯葡胺注射液 注射用血塞通(冻干) 局灶性脑缺血 神经功能评分 脑梗死面积 生化指标 病理改变 

分 类 号：R285.5[医药卫生—中药学]