Identification and characterization of murine adipose tissue-derived somatic stem cells of Shenque(CV8)acupoint  

作　　者：Yu-Hui Hao Zhi-Zhen Liu Hong Zhao Lei Wang Ajab Khan Jian-Bin Mu Yu-Fei Wang Li-Hong Yang Ran Zhou Jun Xie 

机构地区：[1]Shanxi Key Laboratory of Birth Defect and Cell Regeneration,Shanxi Medical University,Taiyuan,Shanxi 030001,China [2]Department of Basic Medicine,Xinzhou Vocational and Technical College,Xinzhou,Shanxi 034000,China [3]Laboratory of Malaria and Vector Research,National Institute of Allergy and Infectious Diseases,National Institutes of Health,Bethesda,MA 20852,USA [4]Basic Medical College,Shanxi University of Chinese Medicine,Jinzhong,Shanxi 030619,China.

出　　处：《Chinese Medical Journal》2021年第22期2730-2737,共8页中华医学杂志（英文版）

基　　金：the National Natural Science Foundation Projects(No.81671462);Shanxi Natural Science Foundation Projects(No.201901D211315 and 201901D111184);Science and Technology Develop-ment Program of Xinzhou(No.20180103).

摘　　要：Background:Shenque(CV8)acupoint is located on the navel and has been therapeutically used for more than 2000 years in Traditional Chinese Medicine(TCM).However,clinical research on the underlying therapeutic molecular mechanisms of the CV8 acupoint lags far behind.This study aimed to study the mechanisms of umbilical acupoint therapy by using stem cells.Methods:The morphological characteristics of CV8 acupoint were detected under a stereomicroscope using hematoxylin and eosin(H&E)staining.Oil Red,Masson,and immunohistochemical staining on multi-layered slices were used to identify the type of cells at the CV8 acupoint.Cell proliferation was measured by a cell counting kit-8(CCK-8)method.Flow cytometry and immunohistochemistry were used for cell identification.Induced differentiation was used to compare the differentiation of cells derived from CV8 acupoint and non-acupoint somatic stem cells into other cell types,such as osteogenic,adipogenic,and neural stem cell-like cells.Results:Morphological observations showed that adipose tissues at the linea alba of the CV8 acupoint in mice had a mass-like distribution.Immunohistochemical staining confirmed the distribution of stem cell antigen-1(Sca-1)positive cells in the multi-layered slices of CV8 acupoint tissues.Cells isolated from adipose tissues at the CV8 acupoint exhibited high expression of Sca-1 and CD44 and low expression of CD31 and CD34,and these cells possessed osteogenic,adipogenic,and neurogenic stem cell-like cell differentiation ability.The cell proliferation(day 4:0.5138±0.0111 vs.0.4107±0.0180,t=8.447,P=0.0011;day 5:0.6890±0.0070 vs.0.5520±0.0118,t=17.310,P<0.0001;day 6:0.7320±0.0090 vs.0.6157±0.0123,t=13.190,P=0.0002;and day 7:0.7550±0.0050 vs.0.6313±0.0051,t=42.560,P<0.0001),adipogenic([9.224±0.345]%vs.[3.933±1.800]%,t=5.000,P=0.0075),and neurogenic stem cell-like cell differentiation(diameter<50 mm:7.2000±1.3040 vs.2.6000±0.5477,t=7.273,P<0.0001;diameter 50–100 mm:2.6000±0.5477 vs.1.0000±0.7071,t=4.000,P=0.0039;and diameter>100 mm:2

关 键 词：Shenque(CV8)acupoint Non-acupoint site NAVEL Adipose tissue Somatic stem cells Stem cell antigen-1(Sca-1) Umbilical therapy 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]