OsEDM2L mediates m^(6)A of EAT1 transcript for proper alternative splicing and polyadenylation regulating rice tapetal degradation  

作　　者：Kun Ma Jingluan Han Zixu Zhang Heying Li Yanchang Zhao Qinlong Zhu Yongyao Xie Yao‐Guang Liu Letian Chen 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro‐Bioresources,South China Agricultural University,Guangzhou 510642,China [2]College of Life Sciences,South China Agricultural University,Guangzhou 510642,China [3]Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510642,China

出　　处：《Journal of Integrative Plant Biology》2021年第11期1982-1994,共13页植物学报（英文版）

基　　金：This work was funded by National Natural Science Foundation of China(32001519,32030080);The Major Program of Guangdong Basic and Applied Research(2019B030302006);China Postdoctoral Science Fund(2020M672653)。

摘　　要：N^(6)-methyladenosine(m^(6)A) modification affects the post-transcriptional regulation of eukaryotic gene expression, but the underlying mechanisms and their effects in plants remain largely unknown. Here,we report that the N^(6)-adenine methyltransferase-like domain-containing protein ENHANCED DOWNY MILDEW 2-LIKE(OsEDM2 L) is essential for rice(Oryza sativa L.) anther development. The osedm2 l knockout mutant showed delayed tapetal programmed cell death(PCD) and defective pollen development. OsEDM2 L interacts with the transcription factors basic helix-loop-helix 142 and TAPETUMDEGENERATIONRETARDATIONto regulate the expression of ETERNAL TAPETUM 1(EAT1), a positive regulator of tapetal PCD. Mutation of OsEDM2 L altered the transcriptomic m^(6)A landscape, and caused a distinct m^(6)A modification of the EAT1 transcript leading to dysregulation of its alternative splicing and polyadenylation, followed by suppression of the EAT1 target genes OsAP25 and OsAP37 for tapetal PCD. Therefore, OsEDM2 L is indispensable for proper messenger RNA m^(6)A modification in rice anther development.

关 键 词：alternative polyadenylation(APA) alternative splicing(AS) N^(6)-methyladenosine(m^(6)A) programmed cell death(PCD) 

分 类 号：S511[农业科学—作物学]