温度对黑斑侧褶蛙变态时长及PHA-P反应的影响  

Effects of temperature on metamorphosis duration and response patterns to phytohemagglutinin-P in the tadpoles of Pelophylax nigromaculatus

作　　者：张志强[1] 王佳慧 金冰艳 陈利 杨雨晴 刁迎珠 伍亮 ZHANG Zhi-qiang;WANG Jia-hui;JIN Bing-yan;CHEN Li;YANG Yu-qing;DIAO Ying-zhu;WU Liang(College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽农业大学动物科技学院,合肥230036

出　　处：《生态学杂志》2021年第12期4029-4033,共5页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(31170379);安徽农业大学2020年度省级重点教学研究项目(2020jyxm0531);安徽农业大学2020年国家级大学生创新创业训练计划项目创新训练项目(202010364072)资助。

摘　　要：为探究温度对蝌蚪变态时长和免疫功能的影响,在21、23、25和27℃水温条件下,测定了黑斑侧褶蛙(Pelophylax nigromaculatus)37期蝌蚪的变态时长,以及变态后对植物血凝素(phytohemagglutinin,PHA-P)反应的变化。结果表明:体重、体长及体重与体长的比值均无组间差异,21、23℃组的变态时长显著高于25和27℃组;对PHA-P的反应的峰值均出现于注射后3 h,随温度升高呈递增趋势,但组间差异不显著;21℃组注射后3 h显著高于0、9和12 h,23℃组注射后3 h显著高于0和12 h,25和27℃组注射后3和6 h都显著高于0 h;升温使黑斑侧褶蛙的变态时长明显缩短,对PHA-P的反应峰值趋于增加但未前移或延迟,说明发育过程中适当升温并不以降低细胞介导的免疫能力作为代价。To explore the effects of temperature on tadpole metamorphosis duration and immune function,we measured the metamorphosis duration of tadpoles ofPelophylax nigromaculatusat Gonser stage 37 under water temperature of 21,23,25,and 27℃,and compared the variations of response to phytohemagglutinin-P(PHA-P)at metamorphosis completion period among different water temperature treatments.The results showed that there was no significant difference in body mass,snout-vent length,and ratio of body mass to snout-vent length among different water temperature treatments(P>0.05).The metamorphosis duration was significantly higher in 21 and23℃treatments than in 25 and 27℃treatments(P<0.05).The responsiveness to PHA-P all peaked at 3 h after injection for all temperature treatments,with an increasing tendency from 21℃group to 27℃group.There was no significant difference for the maximal PHA-P values(P>0.05).The responsiveness to PHA-P was significantly higher at 3 h after injection than at 0,9 and 12 h after injection under 21℃treatment(P<0.05),but was significantly higher at 3 h after injection than at 0 and 12 h under 23℃treatment(P<0.05).Under 25 and 27℃treatments,the responsiveness to PHA-P was significantly higher at 3 and 6 h after injection than at0 h.Increasing temperature greatly shortened the metamorphosis duration of tadpoles ofP.nigromaculatus.There was an enhanced tendency for the responsiveness to PHA-P,but no forward or delayed responsiveness to PHA-P was observed.Our results indicate that moderately elevated temperature would not reduce cell-mediated immunity during development of tadpoles.

关 键 词：蝌蚪 水温 植物血凝素 发育 

分 类 号：Q958[生物学—动物学]