血清核富集转录体1和微小RNA-206与急性冠脉综合征患者经皮冠状动脉介入术后心肌损伤及预后的相关性分析  

Correlation analysis of NEAT1 and miR-206 with myocardial injury and prognosis in patients with acute coronary syndrome after PCI

作　　者：孙鹏瑜 裴月皓 李馨[1] SUN Peng-yu;PEI Yue-hao;LI Xin(Department of Cardiology,Beijing Daxing District People's Hospital,Beijing 102600,China)

机构地区：[1]北京市大兴区人民医院心血管内科,北京市102600

出　　处：《中国心血管病研究》2021年第12期1119-1124,共6页Chinese Journal of Cardiovascular Research

基　　金：北京市科技计划课题(Z171100001017243)。

摘　　要：目的探究血清核富集转录体1(NEAT1)、微小RNA-206(miR-206)对急性冠状动脉综合征(ACS)患者经皮冠状动脉介入(PCI)术后心肌损伤及主要不良心血管事件(MACE)的预测价值。方法选取2017年1月至2020年1月在本院行PCI手术的急性冠状动脉综合征患者102例为研究对象,根据对患者术后随访6个月是否发生心肌损伤及术后随访12个月是否发生MACE,分为心肌损伤组(32例)和心肌未损伤组(70例),MACE组(24例)和非MACE组(78例)。采用实时荧光定量PCR法检测血清中miR-206和NEAT1水平,采用Pearson法分析miR-206水平与NEAT1水平的相关性,采用受试者工作特征(ROC)曲线评价血清NEAT1和miR-206预测ACS患者PCI术后发生心肌损伤及MACE的价值。结果与心肌未损伤组[(1.02±0.26)、(1.05±0.22)]相比,心肌损伤组血清NEAT1(2.13±0.61)水平较高(P<0.05),血清miR-206(0.52±0.15)水平较低(P<0.05);与非MACE组[(1.24±0.07)ng/ml、(4.06±0.97)U/L、(1.05±0.25)、(1.02±0.24)]相比,MACE组术后肌钙蛋白I(cTnI)[(1.72±1.16)ng/ml]、术后肌酸激酶同工酶(CK-MB)[(5.11±1.12)U/L]、血清NEAT1(2.19±0.65)水平较高(P<0.05),血清miR-206(0.47±0.13)水平较低(P<0.05);ROC曲线显示,血清miR-206水平预测ACS患者PCI术后心肌损伤及MACE的曲线下面积(AUC)分别为0.813、0.824,血清NEAT1水平预测急ACS患者PCI术后心肌损伤及MACE的AUC分别为0.851、0.876,二者联合预测ACS患者PCI术后心肌损伤的AUC为0.908,二者联合预测ACS患者PCI术后MACE的AUC为0.926;心肌损伤组及MACE组患者血清miR-206水平与NEAT1水平均呈负相关(P<0.05)。结论 PCI术后发生心肌损伤及MACE的ACS患者血清NEAT1表达上调,血清miR-206表达下调,miR-206和NEAT1可预测ACS患者PCI术后心肌损伤及MACE,且二者联合预测价值较高。Objective To investigate the predictive values of serum nuclear-enriched abundant transcript 1(NEAT1) and microRNA-206(miR-206) for myocardial injury and adverse cardiovascular events(MACE) in patients with acute coronary syndrome after percutaneous coronary intervention(PCI). Methods A total of 102 patients with acute coronary syndrome who underwent PCI surgery in our hospital from January 2017 to January2020 were selected as the research objects. According to whether myocardial injury occurred after 6 months of follow-up and whether MACE occurred after 12 months of follow-up, they were divided into myocardial injury group(32 cases) and non-myocardial injury group(70 cases), MACE group(24 cases) and non-MACE group(78 cases). Real-time fluorescent quantitative PCR method was used to detect the levels of miR-206 and NEAT1 in serum. Pearson method was used to analyze the correlation between the levels of miR-206 and NEAT1;the receiver operating characteristic(ROC) curve was used to evaluate the value of serum NEAT1 and mi R-206 in predicting myocardial injury and MACE in patients with acute coronary syndrome after PCI. Results Compared with the non-myocardial injury group [(1.02±0.26),(1.05±0.22)], the myocardial injury group had higher serum NEAT1(2.13 ± 0.61) level(P<0.05) and lower serum mi R-206(0.52 ± 0.15) level(P<0.05);compared with the non-MACE group [(1.24±0.07) ng/ml、(4.06±0.97) U/L、(1.05±0.25)、(1.02±0.24)], the MACE group had higher postoperative troponin I(c Tn I) [(1.72 ± 1.16) ng/ml], postoperative creatine kinase isoenzyme(CK-MB) [(5.11 ±1.12)U/L], and serum NEAT1(2.19±0.65) levels(P<0.05) but lower serum mi R-206(0.47±0.13) level(P<0.05).The ROC curve showed that the area under the curve(AUC) of serum mi R-206 level predicting myocardial injury and MACE in patients with acute coronary syndrome after PCI was 0.813 and 0.824, respectively;the AUC of serum NEAT1 level predicting myocardial injury and MACE in patients with acute coronary syndrome after PCI was 0.851 and 0.876, respecti

关 键 词：急性冠脉综合征 经皮冠状动脉介入 核富集转录体1 微小RNA-206 心肌损伤 不良心血管事件 

分 类 号：R541.4[医药卫生—心血管疾病]