复合益生菌对大口黑鲈延后繁殖性能、生理指标及仔鱼成活率的影响  

Effects of compound probiotics on reproductive performance,physiological indices and larval survival rate in largemouth bass Micropterus salmoides

作　　者：王赛赛 朱莉飞[1] 李荣妮 史东杰[1] 孙砚胜[1] 张欣[1] WANG Saisai;ZHU Lifei;LI Rongni;SHI Dongjie;SUN Yansheng;ZHANG Xin(National Engineering Research Center for Freshwater Fisheries, Beijing Fisheries Research Institute, Beijing 100068, China)

机构地区：[1]北京市水产科学研究所国家淡水渔业工程技术研究中心,北京100068

出　　处：《大连海洋大学学报》2021年第6期959-965,共7页Journal of Dalian Ocean University

基　　金：财政部和农业农村部:国家现代农业产业技术体系资助(CARS-46);北京市青年拔尖个人项目(2018000021223ZK34);2020年度渔业生物技术北京市重点实验室建设项目(PT2020-19)。

摘　　要：为研究复合益生菌对大口黑鲈Micropterus salmoides延后繁殖性能、生理指标及仔鱼成活率的影响,试验选取150组亲鱼(雌、雄鱼体质量分别为550~665、500~573 g),随机分成5组,每组设3个平行,分别在基础饲料中添加0、0.1%、0.3%、0.5%、0.7%(均为质量分数,下同)复合益生菌制剂,养殖周期为30 d。结果表明:雌鱼的性腺指数、怀卵量、相对怀卵量随饲料中复合益生菌添加量的增加逐渐增大,并于0.5%添加量时达到峰值,但这3个指标在各组间均无显著性差异(P>0.05);0.3%、0.5%复合益生菌添加组第一批产卵量、受精率、孵化率、仔鱼成活率均高于其他组,其中,仅0.5%添加组仔鱼成活率显著高于对照组和0.1%添加组(P<0.05);产卵前,不同试验组雌鱼肝脏中AKP、ACP活力均显著高于产卵后(P<0.05),且2种酶活力均随饲料中复合益生菌添加量的增加逐渐增强,当添加量为0.5%时2种酶活力均达到峰值,产卵前不同添加组雌鱼肝脏中T-SOD活力无显著性差异(P>0.05),0.3%、0.5%添加组雌鱼血浆中T-SOD活力显著高于其他组(P<0.05)。研究表明,复合益生菌添加量为0.5%时,复合益生菌饲料可显著提高大口黑鲈仔鱼成活率,增强产卵前雌鱼肝脏中AKP、ACP活力及血浆中T-SOD活力,本研究结果可为复合益生菌在大口黑鲈延后繁育工作中的应用提供科学参考。In order to study effects of compound probiotics on the delayed reproductive performance,physiological indices and larval survival rate in largemouth bass Micropterus salmoides,150 pairs of broodstock with body mass of 550-665 g in female and 500-573 g in male were randomly reared in 15 tanks of each 5.0 m×2.7 m×1.0 m,and fed 5 diets supplemented with 0,0.1%,0.3%,0.5%and 0.7%of compound probiotics in the basic diet with 3 replicates at water temperature of below 17.5℃for 30 days in early July.Then,3 d after rearing,the largemouth bass broodstocks were induced to spawn at water temperature warming to 21.5-22.5℃by hormone injection.The results showed that the gonado-somatic index(GSI),fecundity and relative fecundity of female were increased gradually with the increase in compound probiotics dose,with the peak in 0.5%group,without significant difference in GSI,fecundity and relative fecundity among the other test groups(P>0.05).There were higher fertilization rate,hatching rate and larval survival rate in first batch in 0.3%and 0.5%groups than those in other groups,with significantly higher survival rate in 0.5%group than that in control group and 0.1%group(P<0.05).There was gradual increase in activities of alkaline phosphatase(AKP)and acid phosphatase(ACP)in liver of female largemouth bass in groups before spawning,and higher than those after spawning(P<0.05),with the peak AKP and ACP activity in the 0.5%group,without significant difference in the activity of total superoxide dismutase(T-SOD)in liver of female fish among different groups before spawning(P>0.05),significantly higher activity of T-SOD in plasma in 0.3%and 0.5%groups than those in other groups(P<0.05).The significant improvement of the larval survival rate and enhancement of the activities of AKP and ACP in the liver and T-SOD activity in plasma were observed in the female fish in 0.5%group.The finding provides theoretical basis for the application of compound probiotics in the delayed breeding of largemouth bass.

关 键 词：大口黑鲈 复合益生菌 繁殖性能 生理指标 仔鱼成活率 

分 类 号：S961.2[农业科学—水产养殖]