葱白提取物对心肌缺血-再灌注损伤大鼠的保护作用及其机制  

Protective Effects and Its Mechanism of Fistular Onion Bulb on Rats with Myocardial Ischemia-reperfusion Injury

作　　者：田立群[1] 雷杰 祝炜[1] 冯莹[1] 张妍 董莹 柯于鹤[1] TIAN Liqun;LEI Jie;ZHU Wei;FENG Ying;ZHANG Yan;DONG Ying;KE Yuhe(Department of Cardiovascular Medicine,Wuhan NO1 Hospital,Wuhan 430022,China;The First Clinical College of Hubei Unirersity of Chinese Medicine, Wuhan 430060,China)

机构地区：[1]武汉市第一医院心血管内科,武汉430022 [2]湖北中医药大学第一临床学院,武汉430060

出　　处：《医药导报》2022年第1期24-29,共6页Herald of Medicine

基　　金：湖北省自然科学基金资助项目(2018CFC801);湖北省卫健委中医药管理局课题(ZY2019M045)。

摘　　要：目的探讨葱白提取物(FOB)预处理对大鼠心肌缺血-再灌注(I/R)损伤的保护作用及其机制。方法SD成年大鼠随机分为假手术组,I/R组,FOB(小、中、大剂量)(300,600,1200 mg·kg^(-1))+I/R组和硝酸甘油+I/R组,每组10只;结扎左冠状动脉前降支30 min再灌注24 h建立在体心肌I/R损伤模型。多导生理记录仪记录和分析左心室功能变化;酶联免疫吸附测定(ELISA)法检测大鼠血清肌酸激酶(CK)、乳酸脱氢酶(LDH)和肌酸激酶同工酶(CK-MB)水平;Western blotting和实时-聚合酶链反应(RT-PCR)检测心肌Bax和Bcl-2蛋白及mRNA表达水平;免疫荧光检测细胞色素C(Cyt-C)和凋亡酶激活因子1(Apaf-1)的表达。结果与I/R组比较,FOB(小、中、大剂量)+I/R组大鼠心功能均明显改善;CK、LDH和CK-MB浓度降低;Bax蛋白及mRNA表达水平下调,同时Bcl-2蛋白及mRNA表达水平升高;Cyt-C及Apaf-1蛋白表达下降(P<0.05),并存在一定量效关系。结论FOB具有显著改善大鼠心肌I/R损伤及拮抗心肌细胞凋亡的作用,其机制与调节心肌Bax、Bcl-2、Cyt-C和Apaf-1的表达密切相关。Objective To investigate the effect and related mechanism of fistular onion bulb(FOB)on myocardial ischemia-reperfusion(I/R)injury in rats.Methods SD rats were divided into six groups(n=10)using random number table method:sham group,I/R group,FOB(low,medium,high dose 300,600,1200 mg·kg^(-1))+I/R groups and nitroglycerin+I/R group.Myocardial I/R model was established by ligation lasted for 30 minutes and opening lasted for 24 hours of left anterior descending coronary artery.The changes of left ventricular function were recorded and analyzed by polygraph.The ELISA method was used to detect myocardial necrotic markers,including creatine kinase(CK),lactate dehydrogenase(LDH)and creatine kinase-MB(CK-MB).Western blotting and RT-PCR methods were used to detect myocardial protein including Bax and Bcl-2 and its mRNA.The levels of Cyt-C and Apaf-1 in myocardial tissue were detectsd by immunofluorescence.Results Compared with I/R group,FOB(low,medium and high dose)+I/R group could significantly improve cardiac function,CK,LDH and CK-MB concentrations were decreased,Bax protein and mRNA expression levels were down-regulated,Bcl-2 protein and mRNA expression levels were increased,Cyt-c and Apaf-1 protein expressions were decreased(P<0.05)that there was a dose-effect relationship.Conclusion Pretreatment with FOB can significantly improve myocardial I/R injury and antagonize myocardial apoptosis in rats,which is closely related to the regulation of the expression of Bax,Bcl-2,Cyt-c and Apaf-1 in myocardium.

关 键 词：葱白提取物 缺血-再灌注损伤 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学] R972[医药卫生—中医学]