甜菜褐斑病菌分离培养及病程分子鉴定  

Isolation of Cercospora beticola and Molecular Identification of Sugar Beet Leaf Spot Course

作　　者：王录红 周翔 李王胜 兴旺[1,2] 谭文勃 WANG Luhong;ZHOU Xiang;LI Wangsheng;XING Wang;TAN Wenbo(National Beet Medium-term Gene Bank,Heilongjiang University,Harbin 150080;College of Advanced Agriculture and Ecological Environment,Heilongjiang University/Key Laboratory of Sugar Beet Genetics and Breeding,Heilongjiang Province Common College,Harbin 150080)

机构地区：[1]黑龙江大学国家甜菜种质中期库,哈尔滨150080 [2]黑龙江大学现代农业与生态环境学院/黑龙江省普通高等学校甜菜遗传育种重点实验室,哈尔滨150080

出　　处：《中国糖料》2022年第1期60-63,共4页Sugar Crops of China

基　　金：黑龙江省自然科学基金项目(LH2019C057);黑龙江省高校基本科研业务费黑龙江大学专项资金项目(KJCX201920);国家作物种质资源库“甜菜分库运行服务”(NCGRC-2021-017);农业农村部“甜菜种质资源的收集、鉴定、编目、繁种与入库(圃)保存”(1921-26);黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2020014)资助。

摘　　要：为了进行分子辅助抗病育种的相关研究,需建立甜菜生尾孢菌的产孢和发病条件。将分离纯化的甜菜生尾孢菌HL001在西红柿培养基上使用涂抹方法接种,经25℃光照培养72 h可以得到大量分生孢子。用孢子悬浮液喷洒接种健康甜菜叶片,温度28℃、湿度90%,光照培养72 h转入常规培养,接种10 d后叶片开始发病并迅速蔓延到整个植株。本研究得到了有关甜菜生尾孢菌产孢和甜菜褐斑病发病的具体条件,对研究甜菜褐斑病抗性基因和甜菜对病原的免疫反应机理奠定了方法学基础。For molecular assisted breeding for disease resistance,the sporulating and pathogenesis conditions should be established.Cercospora beticola HL001 was inoculated on tomato medium by smear.Lots of conidia were collected after culture in light at 25℃ for 72 hours.Inoculating healthy beet folia with conidial suspension,after which the folia were cultured in light at 28℃ and 90% humidity for 72 hours.Then the plants were transferred to routine culture and the leaf spots arose ten days after inoculation.The detail conditions for Cercospora beticola sporulation and sugar beet leaf spot occurrence were obtained.These results lay a foundation of methodology for research on disease-resistant genes and immune response mechanism in sugar beet.

关 键 词：甜菜 褐斑病 甜菜生尾孢菌 分离 培养 分子鉴定 

分 类 号：S435.663[农业科学—农业昆虫与害虫防治]