山东省部分地区水貂养殖场户貂源冠状病毒和新型冠状病毒认知调查及病原检测  

Investigation on Awareness of Mink Farmers Concerning Mink Coronavirus and SARS-COV-2 and Detection of Relevant Pathogens in Shandong Province

作　　者：刘砚涵 党安坤[1] 徐鸿[1] 张栋[1] 李玉杰[1] 蔺晓月 兰邹然[1] 王贵升[1] Liu Yanhan;Dang Ankun;Xu Hong;Zhang Dong;Li Yujie;Lin Xiaoyue;Lan Zouran;Wang Guisheng(Shandong Provincial Center for Animal Disease Control,Jinan,Shandong 250100,China)

机构地区：[1]山东省动物疫病预防与控制中心,山东济南250100

出　　处：《中国动物检疫》2022年第1期37-41,共5页China Animal Health Inspection

基　　金：山东省现代农业产业技术体系特种经济动物创新团队项目(SDAIT-21-05)。

摘　　要：国外水貂感染新型冠状病毒(SARS-CoV-2)遭扑杀事件引起了国内水貂养殖业关注。为了解SARS�CoV-2对山东省水貂养殖场户的影响,通过问卷调查方法了解水貂养殖场户对貂源冠状病毒和SARS-CoV-2的认知情况,同时采集样本应用双重荧光定量PCR方法进行核酸检测,确定水貂中两种病毒的感染状况。结果显示:47.83%的养殖场户对国外水貂感染SARS-CoV-2被扑杀事件有所了解,36.23%的养殖场户认为该事件会对水貂养殖业带来影响,其中10.14%的养殖场户对水貂进行过SARS-CoV-2核酸检测;84.06%的养殖场户认为貂源冠状病毒不会感染人,57.49%认为国外水貂感染SARS-CoV-2的主要途径是通过养殖场其他动物(如犬猫等)感染,仅0.97%认为貂源冠状病毒与SARS-CoV-2存在一定关系,更多的(52.17%)是不清楚;310份样品的双重荧光定量PCR检测结果均为阴性。结果表明,山东省水貂养殖场户普遍意识到貂源冠状病毒与SARS�CoV-2相关性很小,其对水貂养殖业影响不大。但部分养殖场户对这两种病毒及其对水貂养殖业的潜在威胁尚缺乏足够认知,因此需要加强对养殖场户的认知宣传和病毒监测。The stamp-out of minks infected with novel coronavirus(SARS-COV-2)in other countries has been concerned by the stakeholders in mink industry in China.In order to identify the impact of SARS-COV-2 on mink households/farms in Shandong Province,the awareness of farmers concerning mink coronavirus and SARS-COV-2 was investigated by means of questionnaire,and samples were collected to detect nucleic acids by duplex fl uorescence quantitative PCR to identify the prevalence of the two kinds of viruses in minks.The results showed that 47.83%farmers knew about the said event,36.23%farmers believed that it would have an impact on the industry,and 10.14%farmers carried out SARS-COV-2 detection;84.06%farmers considered that human could not be infected with mink coronavirus,57.49%farmers regarded that minks were infected with SARS-COV-2 mainly through contact with other animals(for example,dogs,cats,etc.)in farms,only 0.97%farmers argued that the two viruses were related to some extent,while more farmers(52.17%)knew little;in addition,310 samples were all negative as tested by duplex fl uorescence quantitative PCR.It was concluded that the farmers generally realized that the two viruses were very slightly related and posed little impact on mink industry.However,awareness activities and virus monitoring should be strengthened as some farmers still knew little about the potential threat of the two viruses to the industry.

关 键 词：冠状病毒 新型冠状病毒 水貂 认知调查 生物安全 

分 类 号：S851.3[农业科学—预防兽医学]