稗子用于微生物源生物除草剂筛选的研究  

Studies on Echinochloa crusgalli for the screening of microbial herbicides

作　　者：张志刚[1] 王开梅[1] 吴兆圆 万中义[1] 方伟[1] ZHANG Zhi-gang;WANG Kai-mei;WU Zhao-yuan;WAN Zhong-yi;FANG Wei(Hubei Biopesticide Engineering Research Center/The Branch Center of Biopesiticide,Hubei Agricultural Scientific and Technological Innovation Center,Wuhan 430064,China)

机构地区：[1]湖北省生物农药工程研究中心/湖北省农业科技创新中心生物农药分中心,武汉430064

出　　处：《湖北农业科学》2021年第24期86-89,共4页Hubei Agricultural Sciences

基　　金：国家重点研发计划重点专项(2017YFD0201301-4);湖北省农业科技创新中心创新团队项目(2019-620-000-001-27)。

摘　　要：以马唐[Digitaria sanguinalis(L.)Scop.]作为参照靶标,使用水琼脂表面涂布微孔板法及小型盆栽法对稗子[Echinochloa crusgalli(L.)Beauv]的筛选敏感性进行试验。使用微孔板法对化学除草剂标准样品、真菌及放线菌除草剂标准菌株的发酵液进行了除草活性比较;使用小型盆栽法对真菌及放线菌除草剂标准菌株的发酵液进行了除草活性比较。结果表明,微孔板法中稗子的敏感性差,尤其不适用于针对稗子的微生物源生物除草筛选;小型盆栽法中稗子与马唐的敏感度相近,适用于针对稗子的微生物源除草筛选,具有稳定高、抗杂菌污染强、操作简单等突出优点;针对稗子用于除草剂筛选中敏感性低的缺点可以通过选择适当苗龄的方法加以优化。Digitaria sanguinalis as a reference target,the sensitivity of Echinochloa crusgalli was tested by 96-well plate assay with agar solution and small potted method.The comparison of herbicidal activities of herbicide standard compound,the fermentation broth of herbicide standard strain from fungi and actinomycetes was carried out by 96-well plate assay;The comparison of herbicidal activi⁃ties of the fermentation broth of standard strain of fungi and actinomycetes was carried out by small potted method.The results showed that the sensitivity of Echinochloa crusgalli in 96-well plate assay was poor,especially not suitable for the screening of microbial herbi⁃cidal against Echinochloa crusgalli;The sensitivity of Echinochloa crusgalli and Digitaria sanguinalis in small potted method was simi⁃lar,which was suitable for the microbial herbicide screening against Echinochloa crusgalli,with the outstanding advantages of high stability,strong resistance to microbial pollution,simple operation and so on;In view of the low sensitivity of Echinochloa crusgalli in herbicide screening,the method of selecting appropriate seedling stage was optimized.

关 键 词：稗子[Echinochloa crusgalli(L.)Beauv] 除草活性 微生物源生物除草剂 单子叶植物 

分 类 号：S482.4[农业科学—农药学]