高絮凝活性放线菌的鉴定和培养基组分的初步优化  

Screening of high-efficiency bioflocculant-producing actinobacteria and preliminary optimization of medium composition

作　　者：田宇曦 陈凌 闵勇 杨自文 TIAN Yu-xi;CHEN Ling;MIN Yong;YANG Zi-wen(Hubei Biopesticide Engineering Research Centre,Wuhan 430064,China)

机构地区：[1]湖北省生物农药工程研究中心,武汉430064

出　　处：《湖北农业科学》2021年第24期94-97,共4页Hubei Agricultural Sciences

基　　金：湖北省池塘绿色生态养殖技术创新行动;湖北省中央引导地方科技发展专项(2018ZYYD015)。

摘　　要：为了开发用于农村污水治理的高絮凝活性菌株,拟对高岭土有91.9%絮凝率的放线菌ws75382进行16S rDNA鉴定,并利用单因素试验和正交试验对其培养基组分进行优化。经过16S rDNA鉴定表明,放线菌ws75382与Streptomyces cyslabdanicus相似性最高,放线菌ws75382初始培养基组分多,碳氮源组分浓度偏高,优化获得的培养基组分氮源减少了1个成分,并且碳、氮源浓度分别降低了40%和30%,优化后的培养基组分为大豆粉15 g/L、葡萄糖14 g/L、氯化钠8 g/L和碳酸钙3 g/L,在此培养基组分下,ws75382絮凝率可以达100%。In order to develop a strain with high flocculation activity for rural sewage treatment,actinomycete ws75382 with the floccu⁃lation rate 91.9%of kaolin was identified by 16S rDNA,and its medium composition were optimized by single factor test and orthogo⁃nal test.16S rDNA identification showed that actinomycetes ws75382 had the highest similarity with Streptomyces cyslabdanicus.The initial culture medium of actinomycetes ws75382 had many components,and the concentration of carbon and nitrogen sources was higher.The optimized medium composition reduced the nitrogen sources by one component,and the concentrations of carbon and nitro⁃gen sources reduced 40%and 30%,respectively.The optimized culture medium components were soybean powder 15 g/L,glucose 14 g/L,sodium chloride 8 g/L and calcium carbonate 3 g/L,and under the conditions the flocculation rate of kaolin was nealy 100%.

关 键 词：农村污水治理 放线菌 絮凝 菌株鉴定 培养基组分 

分 类 号：X703[环境科学与工程—环境工程]