茎段长度、无糖培养基与环境对樱桃李试管苗生根的影响  

Effects of stem section, sugar–free medium and environment on rooting of Prunus cerasifera Ehrh. plantlet in vitro

作　　者：夏瑞 柴慈江 黄洁萍 董媛媛 岑芬 Xia Rui;Chai Cijiang;Huang Jieping;Dong Yuanyuan;Cen Fen(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300392,China)

机构地区：[1]天津农学院园艺园林学院,天津300392

出　　处：《天津农学院学报》2021年第4期15-18,22,共5页Journal of Tianjin Agricultural University

基　　金：“天津市林果现代农业产业技术体系创新团队”项目(ITTFPRS2018002)。

摘　　要：为完善樱桃李试管苗生根培养技术,本试验研究了不同长度茎段、无糖培养基及露地环境对樱桃李试管苗生根的影响。结果表明:接种长度15~20 mm的樱桃李试管苗茎段的生根率达86.7%,显著高于长度为5~10 mm的短茎段;不含糖的土壤支撑培养基中试管苗的生根率达到90.0%,且其根长显著高于含糖培养基。9月上旬至10月底在露地环境中培养的樱桃李试管苗生根率为83.3%,与培养室中培养的试管苗差异不显著。本项结果将为完善樱桃李试管苗生根培养技术及降低成本提供依据。In order to improve the in vitro rooting technology of Prunus cerasifera Ehrh. plantlet, the effects of different length of stem section, sugar-free medium and field environment on the rooting of Prunus cerasifera Ehrh. plantlet were studied. The results showed that the rooting rate of stem section inoculated in 15-20 mm length of Prunus cerasifera Ehrh. plantlet in vitro was 86.7%, significantly higher than that of stem section inoculated in 15-20 mm length. With the rooting rate of 90.0%, the root length of the plantlet in soil supporting medium without sucrose was significantly higher than that of the plantlet in medium containing sucrose. From the first ten days of September to the end of October, the rooting rate of Prunus cerasifera Ehrh.Plantlet cultured in the field environment was 83.3%, which was not significantly different from that of the plantlet cultured in the culture room. The results will provide the basis for improving the rooting culture technology and reducing the cost.

关 键 词：樱桃李 茎段 糖 露地 瓶内生根 

分 类 号：S604.3[农业科学—园艺学] S723.123.6