基于Au增强的Mn_(3)O_(4)/Au复合纳米酶的高选择性检测特丁基对苯二酚  

Highly Selective Detection of TBHQ Based on Au-enhanced Mn_(3)O_(4)/Au Composite Nanozyme

作　　者：孟冬玲[1] 王维刚[1] 温佳琦 严俊[1] 李秋兰 周芸[1] 陈志燕[1] MENG Dongling;WANG Weigang;WEN Jiaqi;YAN Jun;LI Qiulan;ZHOU Yun;CHEN Zhiyan(China Tobacco Guangxi Industrial Co.,Ltd.,Nanning 530001;Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500)

机构地区：[1]广西中烟工业有限责任公司,广西南宁530001 [2]昆明理工大学生命科学与技术学院,云南昆明650500

出　　处：《分析科学学报》2021年第6期741-746,共6页Journal of Analytical Science

摘　　要：本研究制备了具有高模拟酶活性的Mn_(3)O_(4)/Au复合纳米酶,该酶可用于催化特丁基对苯二酚(TBHQ)氧化生成红色醌类化合物,基于此原理建立了香精香料中TBHQ的快速比色检测方法。米氏方程动力学模型研究结果表明,Mn_(3)O_(4)/Au复合纳米酶的催化活性远高于Mn_(3)O_(4)和辣根过氧化物酶(HRP)。此外,研究表明金纳米粒子(Au NPs)的加入通过提升电子转移效率,可显著提高复合纳米酶的模拟酶活性。在最优条件下,方法在5~200 mg/kg范围内呈现良好的线性,对TBHQ的检出限为0.6 mg/kg,实际香精样品中TBHQ的加标回收率在93.6%~102.2%范围内,相对标准偏差<5.2%。实际样品中存在的共存物质和其它结构类似的合成酚类抗氧化剂,对所建立的方法几乎无干扰作用,其检测结果也完全可以满足国家标准中对于TBHQ的检测需求。In this study,a Mn_(3)O_(4)/Au composite nanozyme with high mimic enzyme activity was prepared,which can be used to catalyze the oxidation of TBHQ to produce red quinone compound.Based on this principle,a rapid colorimetric detection method for TBHQ in flavors and fragrances was established.The results of the Michaelis equation kinetic model showed that the catalytic activity of the Mn_(3)O_(4)/Au composite nanozyme is much higher than that of Mn_(3)O_(4) and horseradish peroxidase.In addition,the studies reveled that the addition of Au NPs could significantly increase the mimic enzyme activity of the composite nanozyme by improving the electron transfer efficiency.Under optimal conditions,the method showed good linearity in the range of 5-200 mg/kg,the detection limit of TBHQ was 0.6 mg/kg,and the recoveries of TBHQ in actual flavor samples were in the range of 93.6%-102.2%(RSD<5.2%).The co-existing substances in the actual samples and other synthetic phenolic antioxidants with similar structures have almost no interference,and its detection results can fully meet the detection requirements for TBHQ in national standards.

关 键 词：特丁基对苯二酚 纳米酶 高选择性 Mn_(3)O_(4) 

分 类 号：O503[理学—物理]