光敏剂溴化5,10,15,20-四(4-吡啶基,N-丙基胺)对神经母细胞瘤细胞系SH-SY5Y杀伤作用研究  

Study on the Killing Effect of Photosensiter TPPPA on SH-SY5Y Neuroblastoma Cells

作　　者：郭燕 肖勃 陈絮 陆周霞 万洪志 肖雁[2] 肖竦 GUO Yan;XIAO Bo;CHEN Xu;LU Zhouxia;WAN Hongzhi;XIAO Yan;XIAO Song(Department of Chemistry,Basic Medical College,Guizhou Medical University,Guiyang 550025;Key Laboratory of Molecular Biology,Guizhou Medical University,Guiyang 550001)

机构地区：[1]贵州医科大学基础医学院化学教研室,贵州贵阳550025 [2]贵州医科大学分子生物学重点实验室,贵州贵阳550001

出　　处：《分析科学学报》2021年第6期758-764,共7页Journal of Analytical Science

基　　金：贵州省科技厅项目(No.[2018]5779-15);贵州省卫生健康委科技基金(No.2020-1-249)。

摘　　要：合成并研究卟啉衍生物溴化5,10,15,20-四(4-吡啶基,N-丙基胺)(TPPPA)的光敏性质,研究其对神经母细胞瘤细胞系SH-SY5Y杀伤作用。首先测定TPPPA的理化性质,检测其在光照条件下单线态氧的产生情况。光动力作用下观察TPPPA在神经母细胞瘤细胞系SH-SY5Y细胞内的荧光成像,研究细胞内产生活性氧(ROS)的变化,MTT法检测细胞存活率,流式细胞仪检测细胞凋亡。实验结果表明:与5,10,15,20-四(4-吡啶基)卟啉(H_(2)TPyP)比较,带正电荷的TPPPA水溶性较好。TPPPA受光激发能发射明亮的红色荧光,光照条件下单线态氧的产生与光照时间成正比;光动力作用下逐步转移集中于细胞核;产生的ROS随着光照时间延长而增加;对细胞有明显的杀伤作用,具有理想光敏剂的特点。In this paper,porphyrin derivative bromine of 5,10,15,20-tetra(4-pyridyl,N-propyl ammonia)(TPPPA) was synthesized,and its photosensitivity and killing effect on neuroblastoma cell line SH-SY5 Y were studied.Firstly,the physicochemical properties of TPPPA were determined,and singlet oxygen production of TPPPA under light condition was investigated.Fluorescence imaging of TPPPA in SH-SY5 Y cells was observed.The changes of reactive oxygen species(ROS) generated in cells was detected.The killing effect of TPPPA on SH-SY5 Y cells under photodynamic action was determined by MTT assay and flow cytometry analysis.TPPPA can emit bright red fluorescence under light excitation,and the production of singlet oxygen under light condition is proportional to the light time.TPPPA can gradually transfer and concentrate in the cell nucleus under photodynamic action.Intracellular ROS production increases with the extension of light duration,and TPPPA has an obvious killing effect on cells.The above results indicates that TPPPA has the characteristics of an ideal photosensitizer.

关 键 词：卟啉衍生物 神经母细胞瘤 活性氧 光动力杀伤 荧光成像 

分 类 号：O657.3[理学—分析化学]