七氟醚对肾脏缺血再灌注损伤大鼠肾功能及内皮型一氧化氮合酶的影响  

Effects of sevoflurane on renal function and endothelial nitric oxide synthase of rats with renal ischemia reperfusion injury

作　　者：孙燕婷 吴畏 吴思思 薛荣亮[1] SUN Yanting;WU Wei;WU Sisi;XUE Rongliang(Department of Anesthesiology,the Second Affiliated Hospital of Xi′an Jiaotong University,Xi′an,Shaanxi,710004;Department of Anesthesiology,Baoji High-tech People′s Hospital in Shaanxi Province,Baoji,Shaanxi,721013;Department of Anesthesiology,Baoji Central Hospital of Shaanxi Province,Baoji,Shaanxi,721000)

机构地区：[1]西安交通大学第二附属医院麻醉科,陕西西安710004 [2]陕西省宝鸡高新人民医院麻醉科,陕西宝鸡721013 [3]陕西省宝鸡中心医院麻醉科,陕西宝鸡721000

出　　处：《实用临床医药杂志》2021年第24期104-108,共5页Journal of Clinical Medicine in Practice

基　　金：陕西省宝鸡市卫生健康委员会科研计划立项课题(2019-63)。

摘　　要：目的探讨七氟醚(Sev)对肾脏缺血再灌注损伤(IRI)大鼠肾功能、肾小管坏死率及内皮型一氧化氮合酶(eNOS)的影响。方法将45只健康大鼠随机分为MB组(假手术组)、YB组(IRI模型组)、ZL组(Sev+IRI),每组15只。比较3组大鼠血尿素氮(BUN)、血清肌酐(Cr)水平,肾脏组织形态学表现,肾小管坏死率评分,eNOS、诱导型一氧化氮合酶(iNOS)阳性表达以及肾小管上皮细胞凋亡率。结果与MB组大鼠相比,YB组、ZL组大鼠4、12、24 h的BUN、Cr水平均升高,且YB组大鼠12、24 h的BUN、Cr水平均高于ZL组,差异有统计学意义(P<0.05)。MB组大鼠肾脏组织细胞排列整齐、细胞间距均匀;YB组大鼠肾小管有扩张表现,组织排列混乱,细胞扁平且多坏死,病变处为片状,间质充血明显,出现中粒细胞侵润;ZL组上述病灶范围、充血等病理均有一定改善。YB组、ZL组大鼠4、12、24 h肾小管Paller评分均高于MB组,且YB组大鼠4、12、24 h肾小管Paller评分均高于ZL组,差异均有统计学意义(P<0.05)。与MB组相比,YB组大鼠肾组织eNOS强阳性表达降低,iNOS强阳性表达增强,差异有统计学意义(P<0.05);与YB组相比,ZL组大鼠肾组织eNOS强阳性表达增强,iNOS强阳性表达降低,差异有统计学意义(P<0.05)。YB组、ZL组大鼠肾组织中肾小管上皮细胞凋亡率高于MB组,且YB组大鼠肾组织中肾小管上皮细胞凋亡率高于ZL组,差异有统计学意义(P<0.05)。结论Sev干预可改善肾脏IRI大鼠的肾功能,降低肾小管坏死率,对肾脏起保护作用,其机制可能与上调eNOS表达有关。Objective To investigate the effects of sevoflurane(Sev) on renal renal function, renal tubular necrosis rate and endothelial nitric oxide synthase(eNOS) of rats with renal ischemia reperfusion injury(IRI). Methods Forty-five healthy rats were randomly divided into MB group(sham operation group), YB group(IRI model group) and ZL group(SEV plus IRI), with 15 rats in each group. The levels of blood urea nitrogen(BUN) and serum creatinine(Cr), expression of renal histomology, score of renal tubular necrosis rate, positive expressions of eNOS and inductive nitric oxide synthase(iNOS) and apoptosis rate of renal tubular epithelial cells were compared among the three groups. Results Compared with the MB group, the levels of BUN and Cr at 4, 12 and 24 h in the YB group and the ZL group increased significantly, and the levels of BUN and Cr at 12 and 24 h in the YB group were significantly higher than those in the ZL group(P<0.05). In the MB group, the renal tissue cells were arranged neatly and the spacing of cells was uniform. In the YB group,the renal tubules showed dilatation,disorganized tissues,flat cells with multiple necrosis,flaky lesions,obvious interstitial hyperemia and granulocyte invasion. In the ZL group,the lesion range and hyperemia were improved. The Paller scores of renal tubules at 4,12 and 24 h in the YB and ZL groups were significantly higher than that in the MB group,and the Paller scores of renal tubules at 4,12 and 24 h in the YB group were significantly higher than that in the ZL group( P < 0. 05).Compared with the MB group,the positive expression of eNOS was decreased while the positive expression of i NOS was increased in the YB group,and there were significant differences( P < 0. 05).Compared with the YB group,the positive expression of eNOS was increased while the positive expression of i NOS was decreased in the ZL group,and there were significant differences( P < 0. 05).The apoptosis rates of renal tubular epithelial cells in the YB group and the ZL group were significantly higher than that

关 键 词：七氟醚 缺血再灌注损伤 肾功能 内皮型一氧化氮合酶 组织病理学 肾小管坏死率 

分 类 号：R692[医药卫生—泌尿科学] R614[医药卫生—外科学]