僵蚕水溶性物质指纹图谱及共有峰识别研究  

A study on fingerprint and common peaks identification of water-soluble components in Bombyx Batryticatus

作　　者：王斯琦 张露蓉[1] 陈伟[1] 潘韵芝 周峰[2] 苏子衡 程军平 WANG Siqi;ZHANG Lurong;CHEN Wei;PAN Yunzhi;ZHOU Feng;SU Ziheng;CHENG Junping(Suzhou City Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Traditional Chinese Medicine,Suzhou,Jiangsu,215000;the Fifth People′s Hospital of Suzhou City in Jiangsu Province,Suzhou,Jiangsu,215000)

机构地区：[1]南京中医药大学附属苏州市中医医院,江苏苏州215000 [2]江苏省苏州市第五人民医院,江苏苏州215000

出　　处：《实用临床医药杂志》2021年第23期53-57,共5页Journal of Clinical Medicine in Practice

基　　金：江苏省姑苏卫生人才项目(GSWS2019069,GSWS2020094);江苏省药学会-天晴医院药学基金科研项目(Q202027);江苏省苏州市科技局民生科技-科技示范工程项目(SS202011)。

摘　　要：目的采用液相色谱串联质谱(LC-MS/MS)技术对僵蚕水溶性物质中化学成分进行定性分析,建立僵蚕的指纹图谱和共有峰识别的分析方法。方法采用Acclaim PA 25μm C;色谱柱(4.6 mm×250.0 mm),流动相A和B分别为0.2%磷酸溶液和乙腈,流速为0.2 mL/min,柱温为30℃,检测波长为265 nm。采用电喷雾离子源正负离子模式,检测LC-MS/MS获得的特征峰的分子量和分子碎片,并进行特征峰结构指认。结果僵蚕水溶性成分的指纹图谱发现19个共有峰,通过LC-MS/MS识别出其中10个共有峰。10批不同产地的僵蚕药材指纹图谱的相似度评价结果显示,相似度值为0.985~1.000。结论本研究建立的基于LC-MS/MS的僵蚕指纹图谱分析方法具有选择性好、专属性强和灵敏度高等优点,可以为僵蚕药材的质量评价提供技术参考。Objective To qualitatively analyze the water-soluble components in Bombyx Batryticatus by liquid chromatography tandem mass spectrometry(LC-MS/MS) technique and establish the analytical methods of fingerprint and common peaks identification. Methods The column was Acclaim PA 25 μm C;column(4.6 mm×250.0 mm), the mobile phase A and B were 0.2 % phosphoric acid solution and acetonitrile respectively, the flow rate was 0.2 mL/min, the column temperature was 30 ℃, and the detection wave length was 265 nm. An electrospray ionization in both negative and positive ion modes was used to detect the molecular weight and fragment molecular of characteristic peaks obtained by LC-MS/MS, and characteristic peaks were identified. Results A total of 19 common peaks were found in the fingerprints of water-soluble components of Bombyx Batryticatus, and 10 of them were identified by LC-MS/MS. The similarity evaluation results of fingerprints of 10 batches of Bombyx Batryticatus from different producing areas showed that the similarity was between 0.985 and 1.000. Conclusion In this study, the method of fingerprint analysis based on LC-MS/MS is characterized by high selectivity, specificity and sensitivity, which can provide technical reference for quality evaluation of Bombyx Batryticatus.

关 键 词：僵蚕 水溶性物质 指纹图谱 共有峰识别 液相色谱串联质谱 

分 类 号：R282[医药卫生—中药学] R284[医药卫生—中医学]