新生儿窒息患儿早期肠道菌群特征研究  

Characteristics of early intestinal flora in neonatal asphyxia

作　　者：张华婷 尹迪 陈一欢[1] 龚小慧[1] 裘刚[1] 胡勇[1] ZHANG Huating;YIN Di;CHEN Yihuan;GONG Xiaohui;QIU Gang;HU Yong(Department of Neonatology,Shanghai Children's Hospital,Shanghai Jiaotong University,Shanghai 200062,China)

机构地区：[1]上海交通大学附属儿童医院、上海市儿童医院新生儿科,上海200062

出　　处：《中国中西医结合儿科学》2021年第6期485-488,F0003,共5页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：上海市卫生健康委员会卫生行业临床研究专项课题(201940329)。

摘　　要：目的分析比较新生儿窒息患儿粪便中肠道菌群特征,探讨其临床意义。方法选取2019年1~6月在上海交通大学附属儿童医院住院治疗的新生儿窒息患儿7例为研究对象,为窒息组;同期选取我院健康新生儿11例为正常对照组。通过16S rRNA分析其肠道微生态差异。结果新生儿窒息患儿菌群多样性指数较高,其优势菌则为梭菌属、韦荣球菌属、月形单胞菌属、厌氧棍状菌属以及包希菌属。而对照组新生儿以孪生球菌属、颗粒链球菌、肉杆菌科属、嗜血菌属、巴斯德菌科属为优势菌。结论本研究证实了两组新生儿肠道菌群优势定植菌存在显著差异,窒息可能作为影响因素干扰新生儿早期肠道菌群的定植,可能进一步成为窒息患儿胃肠功能紊乱的原因之一。Objective To investigate the characteristics and explore the clinical significance of intestinal flora in asphyxiated neonates.Methods Seven neonates with asphyxia were chosen from Shanghai Children's Hospital as asphyxia group,and 11 healthy neonates were included in the control group.The 16S rRNA sequencing was used to study the difference in intestinal micro-ecology in neonates with asphyxia.Results The diversity index of flora in the neonates with asphyxia was higher,and the dominant bacteria were Clostridium,Veillonella,Lunamonomonas,Anaerobic Sclerotium,and Boschia.Those in the neonates of the control group were Gemini,Streptococcus granulosus,Sarcobacteria,Haemophilus,and Pasteuraceae.Conclusion This study has confirmed that there are significant differences in the intestinal flora between the two groups.Asphyxia may play an important role in the colonization of early intestinal flora in neonatal asphyxia,and may further become a cause of gastrointestinal dysfunction.

关 键 词：新生儿窒息 肠道微生物 高通量测序 

分 类 号：R722.12[医药卫生—儿科]