花生胚小叶再生体系的建立  

Establishment of Regeneration System for Embryo Leaflets of Peanut

作　　者：马倩 马莹莹 高古腔 朱树良 屈成鑫 巩方平 任锐 李忠峰 马兴立[1] 张幸果[1] 殷冬梅[1] Ma Qian;Ma Yingying;Gao Guqiang;Zhu Shuliang;Qu Chengxin;Gong Fangping;Ren Rui;Li Zhongfeng;Ma Xingli;Zhang Xingguo;Yin Dongmei(College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南农业大学农学院,河南郑州450046

出　　处：《山东农业科学》2021年第12期17-24,共8页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(U1704232);河南省产业技术体系项目(S2012-05-G03)。

摘　　要：本研究选用不同花生品种远杂9102、丰花2号和花814的胚小叶为外植体,通过分析不同激素配比、不同基因型诱导不定芽的差异,确定了适宜花生胚小叶不定芽诱导、继代培养的最佳培养基,经伸长和生根培养,最终建立了花生胚小叶的离体再生体系。结果表明,6-BA和NAA组合诱导效果优于TDZ,最佳不定芽诱导培养基为M519+4.5mg/L6-BA+0.2mg/LNAA,丰花2号、远杂9102、花814均有较高的不定芽诱导率;最佳继代培养基为M519+4.0mg/L6-BA,3个基因型品种的芽再生率较高,均超过64%;在1/2MS+0.2 mg/LIBA+0.1mg/LNAA的生根培养基中,丰花2号、远杂9102、花814的生根率依次为73.80%、60.32%、50.66%。本研究初步建立了不依赖于基因型的较广适花生胚小叶再生体系。In this study,the embryo leaflets of different peanut varieties including Yuanza 9102,Fenghua 2 and Hua 814 were used as explants.By analyzing the differences in inducing adventitious buds with different hormones and genotypes,the best media suitable for induction and subculture of adventitious buds of peanut embryo leaflets was determined.After elongation and rooting culture,the in vitro regeneration system of peanut embryo leaflets was established finally.The results showed that the combined induction effect of 6-BA and NAA was better than that of TDZ,and the optimal medium for adventitious bud induction was M519+4.5 mg/L 6-BA+0.2 mg/L NAA.Fenghua 2,Yuanza 9102 and Hua 814 had higher adventitious bud induction rate.The best subculture medium was M519+4.0 mg/L 6-BA,and the bud regeneration rate of the three genotypes were all higher than 64%.In the rooting medium of 1/2 MS+0.2 mg/L IBA+0.1 mg/L NAA,the rooting rates of Fenghua 2,Yuanza 9102 and Hua 814 were 73.80%,60.32%and 50.66%respectively.In this study,a regeneration system of peanut embryo leaflets independent on genotype was established.

关 键 词：花生 胚小叶 不定芽诱导 离体再生体系 

分 类 号：S565.201[农业科学—作物学]