Genome sequencing of the bacterial blight pathogen DY89031 reveals its diverse virulence and origins of Xanthomonas oryzae pv.oryzae strains  

作　　者：Fudan Chen Bingxiao Yan Xiangyu Gong Helin Li Zuhua He 

机构地区：[1]College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China [4]School of Life Sciences,Henan University,Kaifeng 475004,China

出　　处：《Science China(Life Sciences)》2021年第12期2175-2185,共11页中国科学（生命科学英文版）

基　　金：supported by Chinese Academy of Sciences(XDB27040201);the National Natural Science Foundation of China(3181101746)。

摘　　要：The bacterial pathogen Xanthomonas oryzae pv.oryzae(Xoo),belonging to Xanthomonas sp.,causes one of the most destructive vascular diseases in rice worldwide,particularly in Asia and Africa.To better understand Xoo pathogenesis,we performed genome sequencing of the Korea race 1 strain DY89031(J18)and analyzed the phylogenetic tree of 63 Xoo strains.We found that the rich diversity of evolutionary features is likely associated with the rice cultivation regions.Further,virulence effector proteins secreted by the type III secretion system(T3SS)of Xoo showed pathogenesis divergence.The genome of DY89031 shows a remarkable difference from that of the widely prevailed Philippines race 6 strain PXO99A,which is avirulent to rice Xa21,a well-known disease resistance(R)gene that can be broken down by DY89031.Interestingly,plant inoculation experiments with the PXO99A transformants expressing the DY89031 genes enabled us to identify additional TAL(transcription activator-like)and non-TAL effectors that may support DY89031-specific virulence.Characterization of DY89031 genome and identification of new effectors will facilitate the investigation of the rice-Xoo interaction and new mechanisms involved.

关 键 词：DY89031 genome sequencing TAL effectors non-TAL effectors Xanthomonas oryzae pv.Oryzae 

分 类 号：S435.111.47[农业科学—农业昆虫与害虫防治]